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MetabolomicsPhenotypes and Multi—omics Reveal Changes and Molecular Mechanism of Suspension Adaptation of HEK293 Cells Structural Remodelling, Metabolic Reconstruction and Stress Resistance

Human embryonic kidney 293 (HEK293) cells have been successfully adapted from adherent to suspension culture and have been widely applied in both scientific research and the pharmaceutical industry. However, the alterations in cells during the adaptation have not been well described, which raise ...

2025-12-16 | MTBLS13517 | MetaboLights
MetabolomicsMetabolic Response of HEK 293 Cells Exposed to Methylmercury
Not available
ORGANISM(S): Homo sapiens 
ST001776 | MetabolomicsWorkbench
Metabolomics1H HR-MAS NMR Based Metabolic Profiling of Cells in Response to Treatment with a Hexacationic Ruthenium Metallaprism as Potential Anticancer Drug
1H high resolution magic angle spinning (HR-MAS) NMR spectroscopy was applied in combination with multivariate statistical analyses to study the metabolic response of whole cells to the treatment with a hexacationic ruthenium metallaprism [1]6+ as potential anticancer drug. Human ovarian cancer cell...
2015-01-16 | MTBLS156 | MetaboLights
Genomicswhole-genome bisulfite sequencing (BS-seq) of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
Comparison of methylome of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used BS-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51867 | biostudies-arrayexpress
TranscriptomicsTranscriptome of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
Comparison of gene expression profile of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used micrarrays to identify the repertoire of genes differentially expressed upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51863 | biostudies-arrayexpress
GenomicsMethylated DNA immunoprecipitation-sequencing of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
we report the partial methylome (CG-rich regions) of HEK293 cells and HEK293 cells over-expressing the BAHD1 gene (HEK-BAHD1) We used MEDIP-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51864 | biostudies-arrayexpress
TranscriptomicsRole of BACH1 in HEK 293T cells
This SuperSeries is composed of the following subset Series: GSE28050: Expression data from knockdown of BACH1 in HEK 293T cells GSE28051: Genome-wide map of BACH1 binding in HEK293T cells Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-05-12 | E-GEOD-28053 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human monoclonal cell lines with high or low levels of store-operated Ca2+ entry
This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded ...
ORGANISM(S): Homo sapiens 
2007-08-03 | E-GEOD-1309 | biostudies-arrayexpress
MetabolomicsComparative analysis of the adaptation of Staphylococcus aureus to internalization by different types of human non-professional phagocytic host cells (NMR assay)
Staphylococcus aureus is a human pathogen that can cause a wide range of diseases. Although formerly regarded as extracellular pathogen, it has been shown that S. aureus can also be internalized by host cells and persist within these cells. In the present study, we comparatively analyzed survival an...
2015-07-01 | MTBLS102 | MetaboLights
TranscriptomicsBPA impacts on HEK 293 cells
Bisphenol A (BPA) is an environmental endocrine disruptor which has been detected in almost all human bodies. Many studies have implied that BPA exposure is harmful to human health. Previous studies mainly focused on BPA effects on ER-positive cells. Genome-wide impact of BPA on gene regulation in E...
ORGANISM(S): Homo sapiens 
2014-10-10 | E-MTAB-1959 | biostudies-arrayexpress
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