Hek293t cell line

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Proteomics (260)
Unknown (205)
Genomics (192)
Transcriptomics (188)
Metabolomics (8)
Other (3)

Organisms

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Entamoeba histolytica HM-1:IMSS-A (220)
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Leishmania major strain Friedlin (220)
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Methylohalobius crimeensis 10Ki (220)
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BTO:0002181 (2)
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cellular organisms (1)

Organisms

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Repository

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Tissue

Cell culture (118)
Permanent cell line cell (96)
Epithelial cell (22)
Kidney (13)
Early embryonic cell (11)
HEK-293T cell (8)
Fibroblast (5)
Embryonic kidney cell line (4)
Embryonic stem cell (4)
Kidney cell (4)
Brain (3)
Cell suspension culture (3)
HeLa cell (3)
Heart (3)
Cell line (2)
Blood (2)
Macrophage (2)
Primary cell (2)
Stem cell (2)
Blood plasma (1)
Brown adipocyte (1)
Cardiocyte (1)
Cell line cell (1)
Cervical cancer cell line (1)
Colon (1)
Endothelial cell of umbilical vein (1)
HEK-293 cell (1)
HEK-293ET cell (1)
HiPSC cell (1)
MCF-10A cell (1)

Disease

Disease free (18)
Acute leukemia (2)
Breast cancer (2)
Prostate cancer (1)
3-methylglutaconic aciduria type 9 (1)
Amyotrophic lateral sclerosis (1)
Bone cancer (1)
Brain cancer (1)
Cancer (1)
Cervix carcinoma (1)
Charcot-Marie-Tooth disease axonal type 2C (1)
Congenital contractural arachnodactyly (1)
Huntington Disease (1)
Influenza (1)
Lung cancer (1)
Malignant neoplasm of ovary (1)
Marfan syndrome (1)
Mitochondrial metabolism disease (1)
Musculoskeletal system disease (1)
Myelodysplastic syndrome (1)
Neuroblastoma (1)
Polycystic Kidney (1)
Retinitis pigmentosa (1)
Severe acute respiratory syndrome (1)
Thymic carcinoma (1)
Tonne-Kalscheuer syndrome (1)
Type 2 diabetes mellitus (1)
Urinary bladder cancer (1)

Technology Type

Mass Spectrometry (220)
Shotgun proteomics (96)
Affinity purification coupled with mass spectrometry proteomics (83)
Bottom-up proteomics (29)
Gel-based experiment (25)
Data-dependent acquisition (18)
Chemical cross-linking coupled with mass spectrometry proteomics (8)
Data-independent acquisition (6)
Mass spectrometry (5)
SWATH MS (5)
SRM/MRM (3)
Mass spectrometry assay (2)
Top-down proteomics (2)
Affinity proteomics (1)
All-ion fragmentation (1)
MSE (1)
Mass spectrometry imaging (1)

Instrument Platform

Illumina NovaSeq 6000 (18)
Illumina HiSeq 2500 (8)
Illumina HiSeq 2000 (6)
NextSeq 500 (6)
NextSeq 550 (5)
LTQ (5)
Illumina HiSeq 4000 (4)
Orbitrap Fusion Lumos (3)
Illumina MiSeq (2)
LTQ Orbitrap Velos (2)
Orbitrap Exploris 480 (2)
Orbitrap Fusion (2)
Q Exactive (2)
Unknown (1)
E.Z.N.A. Total RNA Kit I (Omega Bio-tek) (1)
MoFlo Legacy cell sorter (Beckman Coulter) (1)
PC (1)
BD FACSARIA III (1)
Fluidigm C1 (1)
Affymetrix Primeview Human Gene Expression Array (1)
GenePix 4000B [Axon Instruments] (1)
Illumina NovaSeq X (1)
Ion Torrent PGM (1)
NA (1)
LTQ (Thermo Scientific instrument model) (1)
LTQ Orbitrap Elite (1)
LTQ Velos (1)
Orbitrap Ascend (1)
Orbitrap Eclipse (1)
Orbitrap Fusion ETD (1)

Publication Date

2022 (52)
2023 (47)
2025 (44)
2021 (38)
2020 (38)
2024 (36)
2015 (33)
2019 (32)
2018 (27)
2014 (25)
2026 (19)
2011 (19)
2013 (17)
2012 (15)
2017 (13)
2016 (12)
2010 (7)
2008 (5)
2009 (2)
2007 (2)

CHEBI ID

CHEBI:16750 (3)
CHEBI:16335 (3)
CHEBI:16704 (3)
CHEBI:85574 (2)
CHEBI:67070 (2)
CHEBI:67066 (2)
CHEBI:67067 (2)
CHEBI:67077 (2)
CHEBI:85568 (2)
CHEBI:85567 (2)
CHEBI:85566 (2)
CHEBI:85565 (2)
CHEBI:66861 (2)
CHEBI:66858 (2)
CHEBI:67068 (2)
CHEBI:72347 (2)
CHEBI:66847 (2)
CHEBI:64536 (2)
CHEBI:64480 (2)
CHEBI:64540 (2)
CHEBI:67064 (2)
CHEBI:67065 (2)
CHEBI:64541 (2)
CHEBI:67076 (2)
CHEBI:66862 (2)
CHEBI:66856 (2)
CHEBI:64424 (2)
CHEBI:20794 (2)
CHEBI:64431 (2)
CHEBI:64524 (2)

Metabolite Name

Guanosine (3)
7-methylguanosine (2)
Uridine (2)
Pseudouridine (2)
Inosine (2)
Cytidine (2)
Adenosine (2)
Uracil (2)
1-methyladenosine (2)
N6-methyladenosine (2)
N6-,O2'dimethyladenosine (1)
N4-Methylcytidine (1)
Lysotracker Red DND-99 (1)
Glycine 13C2-15N (1)
2-Hydroxybutyric acid (AHB) (1)
Carbamoyl Aspartate (1)
Asymmetric dimethylarginine (ADMA) (1)
Alpha-Ketoglutarate (aKG) (1)
S-Adenosyl-L-methionine (SAM) (1)
Gamma-aminobutyric acid (GABA) (1)
Formiminoglutamic acid (FIGLU) (1)
5-Hydroxyindole-3-Acetate (1)
S-Adenosyl-L-Homocysteine (SAH) (1)
P-Aminobenzoate (1)
Trans-urocanic acid (1)
Cis-urocanic acid (1)
Acetyl CoA (1)
3-hydroxyisobutyric acid (1)
H1 (1)
SM C26:1 (1)

First Public Date

2025 (33)
2024 (27)
2023 (22)
2022 (14)
2020 (12)
2019 (12)
2026 (11)
2021 (9)
2018 (8)
2017 (7)
2014 (7)
2016 (1)

Modification

2x(15)N labeled L-lysine (2)
4x(15)N labeled L-arginine (2)
15.994915 (1)
4x(2)H labeled dimethylated residue (1)
57.021464 (1)
71.037114 (1)
N6-dimethyl-L-lysine (1)

Study type

Transcription profiling by array (76)
RNA-seq of coding RNA (40)
ChIP-seq (17)
RNA-seq of total RNA (6)
Cell line - High-throughput sequencing (5)
Other (5)
RNA-seq of non coding RNA (5)
ChIP-chip by tiling array (3)
ATAC-seq (3)
RNA-seq of coding RNA from single cells (3)
Unknown experiment type (2)
RNAi profiling by array (2)
ChIP-chip by array (2)
CLIP-seq (2)
Methylation profiling by array (2)
CUT&RUN (2)
Methylation profiling by high throughput sequencing (2)
Transcription profiling by high throughput sequencing (1)
Baseline (1)
MicroRNA profiling by array (1)
DNA-seq (1)
Proteomic profiling by array (1)
Genotyping by high throughput sequencing (1)

Release Date

2015 (40)
2022 (37)
2021 (31)
2023 (30)
2025 (24)
2020 (24)
2014 (24)
2011 (24)
2018 (21)
2017 (19)
2016 (19)
2013 (19)
2012 (19)
2024 (17)
2019 (15)
2010 (7)
2026 (6)
2008 (6)
2009 (3)
2007 (3)

Lab affiliation

Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands (10)
Department of Cell Biology, Harvard Medical School, Boston MA 02115 USA (6)
Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USA (4)
Institute of Biochemistry 2, Goethe University Frankfurt, Germany (3)
Crick-GSK LinkLabs, UK (2)
Department of Chemistry, Imperial College London, Molecular Research Science Hub, 80 Wood Lane, London W12 0BZ, United Kingdom. The Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom. (2)
Department of Molecular & Integrative Physiology,University of Michigan Medical School, 5325 Brehm Tower, 1000 Wall St., Ann Arbor, MI, 48105 (2)
Département de Biologie Cellulaire, Université de Genève (2)
Institute of Virology - Viral Immunopathology, Faculty of Medicine, Technical University Munich (2)
Massachusetts General Hospital Harvard Medical School (2)
Max Delbruck Center for Molecular Medicine, Robert-Rössle-Str. 10, D-13092 Berlin, Germany (2)
Molecular Biosciences and the Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX USA (2)
Principal Scientist, CSIR- Centre for Cellular and Molecular Biology, Hyderabad, India (2)
Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Associate Editor, ACS Chemical Biology (2)
Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Executive Editor, ACS Chemical Biology (2)
VIB Center for Medical Biotechnology, VIB, Ghent, B9052, Belgium Department of Biomolecular Medicine, Ghent University, Ghent, B9052, Belgium (2)
Wiita Lab, Department of Laboratory Medicine, University of California San Francisco, USA (2)
1. Center for Medical Biotechnology, VIB, Ghent, Belgium 2. Department for Biomolecular Medicine, Ghent University, Ghent, Belgium 3. VIB Proteomics Core, VIB, Ghent, Belgium (1)
1. Center for Medical Biotechnology, VIB, Ghent, Belgium. 2. Department for Biomolecular Medicine, Ghent University, Ghent, Belgium 3. VIB Proteomics Core, VIB, Ghent, Belgium (1)
1. Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA. 2. Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA. (1)
1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA. 2Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA. 3Biological and Biomedical Sciences Program, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. (1)
1VIB-UGent Center for Medical Biotechnology, VIB, 9052 Ghent, Belgium 2Department of Biomolecular Medicine, Ghent University, 9052 Ghent, Belgium (1)
Associate Member, Hematological Malignancies Program St. Jude Children's Research Hospital Memphis-TN UA (1)
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences , Utrecht University, 3584 CH Utrecht, The Netherlands (1)
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht 3584 CH The Netherlands Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH, The Netherlands (1)
COI-NDM (University of Oxford) (1)
CRCM, INSERM, CNRS, Institut Paoli-Calmette, Aix-Marseille University, 27 Boulevard Leï Roure, 13009, Marseille, France (1)
Cancer Biology and Genetics, Wexner Medical Center, The Ohio State University, Columbus, OH, USA (1)
Cardiovascular and Metabolic Diseases, Duke-NUS Medical School, Singapore (1)
Cell Biology, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK. (1)

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Real-world neonicotinoid mixture exposure reveals synergistic cytotoxicity and divergent pathway vulnerabilities in human cell lines

In this study, five human cell lines (A2780, Chang liver, HEK293T, HeLa, and HepG2) were used as models and exposed to mixtures of neonicotinoids (NEOs) and their metabolites. The mixture concentrations were prepared based on human biomonitoring data to reflect the actual detected levels of NEOs ...

2026-06-22 | MTBLS13896 | MetaboLights

Gene expression analysis of HEK293T cells after CHMP5 gene modulation

Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation. In this study, HEK293T cells, silenced for CHMP5 using sh-CHMP5 was compared to genome wide expression changes when compared to cellular transfections with a sh-lucif...

ORGANISM(S): Homo sapiens

2010-07-08 | E-GEOD-17003 | biostudies-arrayexpress

Transcriptomic profiling of HBG gene expression in HEK293T cell line

PRJNA1085296 | ENA

AP-MS analysis using HEK293T cells for stable cell line controls

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

2017-07-19 | MSV000081360 | MassIVE

ATAC-seq of HEK293T cell line

PRJEB20596 | ENA

RNA-seq of HEK293T cell line

PRJEB20597 | ENA

ChIP-sequencing of Human FOXN2 and RFX1 in HEK293T Cells

We have performed ChIP-sequencing analysis on human FOXN2 and RFX1 target sequences in human embryonic kidney HEK293T cells stably expressing Streptavidin-S-FLAG (SFB) triple-tagged proteins. The NGS sequencing were performed on Illumina MiSeq desktop sequencer.

ORGANISM(S): Homo sapiens

2015-01-01 | E-MTAB-3120 | biostudies-arrayexpress

The deafness gene DFNA5 induces programmed cell death through mitochondria and MAPK-related pathways [HEK293T]

The function of DFNA5 remained unknown for a long time, but previous functional studies by Op de Beeck et al. (2011) revealed that DFNA5 induces a growth defect in mutDFNA5- transfected HEK293T cells, as well as other cells, leading to PCD (Op de Beeck et al., 2011). The cell death-inducing capacity...

ORGANISM(S): Homo sapiens

2015-06-24 | E-GEOD-70167 | biostudies-arrayexpress

Effect of SRPK1 knockdown on gene expression of HEK293T cell line

PRJNA1193674 | ENA

RNA seq Analysis of Wild Type and CAMTA1 knockout HEK293T cell line

PRJNA808880 | ENA