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TranscriptomicsHost cell responses to persistent mycoplasmas--different stages in infection of HeLa cells with Mycoplasma hominis
M. hominis adherence, colonisation and invasion of HeLa cells were characterised in a time-course study using microarray-based analysis of the HeLa cell transcriptome: 0 h to monitor the baseline of transcription, 4 h to examine host reactions to mycoplasma attachment, 48 h to capture in addition th...
ORGANISM(S): Homo sapiens 
2014-02-06 | E-GEOD-54698 | biostudies-arrayexpress
ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-12 | MSV000081607 | MassIVE
OtherConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Data from ProteomeXchange, PXD ID: PXD000900. Data file: HeLa_ArgC-Elastase_CID_2.mgf. Published as part of Mol Cell Proteomics. 2014 Apr 2 . From the Abstract: {{i}} ... In an attempt to improve protein sequence coverage and to target regions of proteins that do not generate useful tryptic peptide...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210026998 | GPMDB
Resource Reanalysis 
ProteomicsHeLa Cells Cross-Linked In Vivo versus as a Lysate
HeLa Cells were cross-linked with PIR either in vivo or as a lysate.
ORGANISM(S): Homo sapiens (Human) 
2019-07-12 | PXD013063 | Pride
Pir cross-linker xl-ms 
ProteomicsHeLa lysate sample for DeBruijn decoy experiments (contains three replicates)
HeLa lysate sample for DeBruijn decoy experiments (contains three replicates). The raw files are converted using MSConvert. The target sequence is a Human dataset downloaded from UniProt (ID: UP000005640). The decoy databases are generated using the proposed deBruijn decoy method and random shuffli...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-08-14 | MSV000084207 | MassIVE
TranscriptomicsRIP: miR-202 mimic-transfected HeLa cells vs. negative control
Potential miR-202 targets were identified using a targetome-wide RIP-based microarray. HeLa cells were transfected with either a miR-202 mimic or a scrambled single-stranded RNA negative control. RNA was isolated from total cell lysate prior to IP and from antibody-immobilized Protein G agarose be...
ORGANISM(S): Homo sapiens 
2012-12-19 | E-GEOD-42981 | biostudies-arrayexpress
OtherConfetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.
Data from ProteomeXchange, PXD ID: PXD001258. Experiment: Hela_Try_80pcACN_CID_3xload, file: Hela_Try_80pcACN_CID_3xload_3_20140722.mzXML. Information serves as a correction to Mol Cell Proteomics. 2014 Jun;13(6):1573-84 . From ProteomeXchange: {{i}} Contains corrected HeLa SAX fractionated dataset...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320002604 | GPMDB
Resource Reanalysis 
ProteomicsLC-MS/MS analysis of KA-PARP1 targets in HeLa celly lysate
KA-PARP1 was expressed in HeLa-KO PARP1 cells. Protein targets of KA-PARP1 were determined in cell lysate by using a DTB-modified NAD+analog.
ORGANISM(S): Homo sapiens (Human) 
2024-05-02 | PXD046956 | Pride
Lc-ms/ms Parp1 
TranscriptomicsENO1's RNA-binding sites in Human HeLa cells using eCLIP
eCLIP was performed for ENO1 in HeLa cells, following the protocol described by Van Nostrand et al. (2016). Libraries for six immunoprecipitation and size-matched input controls were produced. In addition, libraries were produced for two no-crosslinking controls. The libraries were sequenced using p...
ORGANISM(S): Homo sapiens 
2020-10-27 | E-MTAB-9031 | biostudies-arrayexpress
TranscriptomicsiCLIP of FXR1-WT and FXR1-V361P in HeLa cells
In the associated paper FXR1 is shown to package exceptionally long mRNAs in the cytoplasm and organizes them into an mRNP network. We performed iCLIP of FXR-WT and its mutant FXR1-V361P in HeLa cells where we knocked down endogenous FXR1 and replaced it with either GFP-tagged WT or V361P-mutant FXR...
ORGANISM(S): Homo sapiens 
2024-11-01 | E-MTAB-13545 | biostudies-arrayexpress
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