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GenomicsHomo sapiens strain:K562/HelaS3
Homo sapiens strain:K562/HelaS3 Transcriptome or Gene expression
PRJNA185007 | ENA
TranscriptomicsmRNA profile of HeLaS3 cell line and AGO2 Knock-Out HeLaS3 subclone
We aimed to identify the effect of AGO2 depletion by genome editing on the mRNA expression profile of human cells. The experimental model used is the human cell line HeLaS3. All copies of AGO2 gene were disrupted by targeted genome editing using ZNF nucleases.
ORGANISM(S): Homo sapiens 
2019-01-28 | E-MTAB-7085 | biostudies-arrayexpress
GenomicsAgo2-bound short RNAs in HeLaS3 cell line
Ago2-bound short RNAs in HeLaS3 cell line
PRJEB4987 | ENA
GenomicsAgo1-bound short RNAs in HeLaS3 cell line
Ago1-bound short RNAs in HeLaS3 cell line
PRJEB5031 | ENA
TranscriptomicsAgo2-bound short RNAs in HeLaS3 cell line
Characterization of AGO2 bound short RNAs from nuclear extracts of HeLaS3 cell line. Nuclei were isolated from HeLaS3 cells; a fraction of Nuclear lysates was harvsted and sequenced as the 'input' samples. The rest of nuclear lysates were Immunoprecipitated using AGO2 monoclonal antibodies or Isotyp...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2084 | biostudies-arrayexpress
TranscriptomicsAgo1-bound short RNAs in HeLaS3 cell line
Characterization of AGO1 bound short RNAs from nuclear extracts of HeLaS3 cell line. Nuclei were isolated from HeLaS3 cells; a fraction of Nuclear lysates was harvsted and sequenced as the input samples. The rest of nuclear lysates were immunoprecipitated using AGO1 monoclonal antibodies or Isotype ...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2129 | biostudies-arrayexpress
TranscriptomicsRNA-seq of AGO2 knock-down HeLaS3 cell line
In order to assess the effect of AGO2 on the transcriptional and post-transcriptional regulation of gene expression, RNA expression was profiled in untreated, CTRL siRNA transfected and AGO2 siRNA transfected HeLaS3 cells
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2128 | biostudies-arrayexpress
GenomicsRNA-seq of AGO2 knock-down HeLaS3 cell line
RNA-seq of AGO2 knock-down HeLaS3 cell line
PRJEB5044 | ENA
GenomicsATAC-seq essays in A549 and HeLaS3 during cell cycle
ATAC-seq essays in A549 and HeLaS3 during cell cycle
PRJNA801844 | ENA
GenomicsMNASE_seq_of_AGO2_Knock_Down_HeLaS3_cells
We knocked down AGO2 protein by RNAi in HeLaS3 human cell line. The current experiment aims at comparing nucleosome positioning in control and AGO2 knock down cells. HeLaS3 cell were cultured in DMEM. Cells were transfected with AGO2 siRNAs (or control siRNA) at day 1. On day 4 MNAse digestion of c...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-1844 | biostudies-arrayexpress
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