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ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range thermal profiling experiment 2
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033650 | Pride
Toxoplasma Thermal proteome profiling Proteomics Parasite Calcium signaling Cetsa Tpp Apicomplexan Systems biology 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: temperature range thermal profiling experiment
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033713 | Pride
Toxoplasma Parasite Calcium signaling Cetsa Tpp Apicomplexan Systems biology Thermal profiling 
ProteomicsPKA R-dependent phosphoproteome of T. gondii treated with the phosphodiesterase inhibitor zaprinast
Toxoplasma gondii is a ubiquitous pathogen of warm-blooded animals and belongs to the phylum of apicomplexan parasites. Apicomplexans cause diseases of high mortality, including toxoplasmosis, malaria, and cryptosporidiosis. Cyclic nucleotide-dependent protein kinases in this divergent family of par...
ORGANISM(S): Toxoplasma gondii RH 
2024-07-12 | PXD044398 | Pride
Toxoplasma gondii T. gondii Phosphoproteomics Parasitology 
ProteomicsPKG-dependent phosphoproteome of T. gondii treated with the phosphodiesterase inhibitor zaprinast
Apicomplexans are ubiquitous parasites of humans and cause diseases including toxoplasmosis, malaria, and cryptosporidiosis. Protein kinase G (PKG) possesses unique features in this phylum of parasites and is a demonstrated drug target. Phosphodiesterase inhibitors increase the concentration of cGMP...
ORGANISM(S): Toxoplasma gondii RH 
2024-07-12 | PXD044361 | Pride
Toxoplasma Pkg Parasite T. gondii Phosphoproteomics Protein kinase g 
ProteomicsT. gondii TIR1 strain temperature range thermal profiling experiment
Toxoplasma gondii is a single-celled eukaryotic parasite that chronically infects a quarter of the global population. In recent years, phenotypic screens have identified compounds that block parasite replication. Unraveling the complexity of pathogenesis and molecular mechanisms and pathways perturb...
ORGANISM(S): Toxoplasma gondii RH 
2023-03-11 | PXD024912 | Pride
Parasite Tpp Cetsa Toxoplasma 
ProteomicsThermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)
Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extra...
ORGANISM(S): Toxoplasma gondii GT1 
2020-07-13 | PXD018810 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa 
ProteomicsIdentification of proteins exhibiting TgDegP2-responsive thermal stability
To broadly explore artemisinin susceptibility in apicomplexan parasites, we used genome-scale CRISPR screens recently developed for Toxoplasma gondii to discover sensitizing and desensitizing mutations. From these screens, we identified the mitochondrial protease DegP2, which appeared gDegP2-respons...
ORGANISM(S): Toxoplasma gondii GT1 
2020-08-28 | PXD019917 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa Artemisinin susceptibility 
ProteomicsThermal proteome profiling of Toxoplasma gondii treated with enhancer 1 (ENH1)
reviously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extrac...
ORGANISM(S): Toxoplasma gondii GT1 
2023-08-21 | PXD018818 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range experiment 1
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033642 | Pride
Toxoplasma Parasite Calcium signaling Cetsa Tpp Systems biology Thermal profiling 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive phosphoproteome
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033765 | Pride
Toxoplasma Parasite Calcium signaling Phosphoproteomics Apicomplexan Systems biology 
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