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TranscriptomicsGenomic analysis of hESC pedigrees enables identification of de novo genetic alterations and determination of the timing and origin of mutational events
Given the association between mutational load and cancer, the observation that genetic aberrations are frequently found in human pluripotent stem cells (hPSCs) is of concern. Prior studies in hiPSCs have shown that deletions and regions of loss-of-heterozygosity (LOH) tend to arise during reprogramm...
ORGANISM(S): Homo sapiens 
2014-04-21 | E-GEOD-49452 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human embryonic stem cells (hESC) line Amicqui-1 derived and maintained on human amniotic epithelial cells
Conventional conditions to maintain human embryonic stem cells (hESC) imply the use of inactive mouse embryonic fibroblast (iMEF) as a feeder layer. However, it has suggested that the culture of hESC on iMEF could be an artifact that does not correspond to the in vitro counterpart of the human epibl...
ORGANISM(S): Homo sapiens 
2022-05-01 | E-MTAB-10347 | biostudies-arrayexpress
TranscriptomicsELABELA Is an Endogenous Growth Factor that Sustains hESC Self-Renewal Via the PI3K/AKT Pathway
ELABELA (ELA) is a peptide hormone required for heart development that signals via the Apelin Receptor (APLNR, APJ). ELA is also abundantly secreted by human embryonic stem cells (hESCs), which do not express APLNR. Here we show that ELA signals in a paracrine fashion in hESCs to maintain self-rene...
ORGANISM(S): Homo sapiens 
2015-09-20 | E-GEOD-71949 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of gene expression patterns responding to TSA treatment during hESC neural differentiation
We report the differential roles of an HDAC inhibitor-TSA during hESC nerual commitment. In the initiation of hESC differentiation, TSA could inhibit the downregulation of pluripotency genes to maintain pluripotency, whereas in the neural commitment stage, TSA could promote neural gene expression to...
ORGANISM(S): Homo sapiens 
2015-03-11 | E-GEOD-66777 | biostudies-arrayexpress
TranscriptomicsHuman embryonic stem cells derived from embryos at different stages of development share similar transcription profiles
We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undiffer...
ORGANISM(S): Homo sapiens 
2011-10-20 | E-GEOD-29625 | biostudies-arrayexpress
TranscriptomicsExpression data from a new FACS approach isolated hESC derived endoderm cells using transcription factors
We show that high quality microarray gene expression profiles can be obtained following FACS sorting of cells using combinations of transcription factors. We use this transcription factor FACS (tfFACS) methodology to perform a genomic analysis of hESC-derived endodermal lineages marked by combinatio...
ORGANISM(S): Homo sapiens 
2011-08-23 | E-GEOD-24135 | biostudies-arrayexpress
TranscriptomicsCharacterization of human embryonic stem cells with features of neoplastic progression
Cultured human embryonic stem (hES) cells can acquire genetic and epigenetic changes that make them vulnerable to transformation. As hES cells with cancer-cell characteristics share properties with normal hES cells, such as self-renewal, teratoma formation and the expression of pluripotency markers,...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-13995 | biostudies-arrayexpress
TranscriptomicsComparing isogenic pairs of hESC and hiPSC lines reveals genetic background and reprogramming method as primary sources of transcriptional variation
The equivalency of human induced pluripotent stem cells (hiPSCs) with human embryonic stem cells (hESCs) remains controversial. Here, we devised a strategy to assess the contribution of clonal growth, reprogramming method and genetic background to transcriptional patterns in hESCs and hiPSCs. Surpri...
ORGANISM(S): Homo sapiens 
2015-10-26 | E-GEOD-73211 | biostudies-arrayexpress
TranscriptomicsBG02 hESC Exposed to Butyrate
To assess the effect of sodium butyrate exposure on human ESC grown without culture support for self-renewal (I.e. without conditioned medium and added bFGF) in support of data generated on H1 hESC - two groups were compared - BG02 culture in feeder conditioned versus on sodium butyrate - in triplic...
ORGANISM(S): Homo sapiens 
2009-03-04 | E-GEOD-15109 | biostudies-arrayexpress
TranscriptomicsExpression data from undifferentiated human embryonic stem cells (hESC) and Day 3.5 mesodermal progenitor (CD326neg CD56+) population
Our understanding of how mesodermal tissue is formed, has been limited by the absence of specific and reliable markers of early mesoderm commitment. We report that mesoderm commitment from human embryonic stem cells (hESC) is initiated by Epithelial to Mesenchymal transition (EMT) as shown by gene e...
ORGANISM(S): Homo sapiens 
2010-05-27 | E-GEOD-21668 | biostudies-arrayexpress
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