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ProteomicsEngineering cell-free systems by chemoproteomic-assisted phenotypic screening.
Phenotypic screening is a valuable tool to both understand and engineer complex biological systems. We demonstrate the functionality of this approach in the development of cell-free protein synthesis (CFPS) technology. Phenotypic screening identified numerous compounds that enhanced protein produc...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2024-07-22 | PXD047986 | JPOST Repository
TranscriptomicsRNA-seq of coding RNA of Pichia pastoris GS115 to determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants
To determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants in Pichia pastoris
ORGANISM(S): Pichia pastoris 
2013-09-12 | E-MTAB-1126 | biostudies-arrayexpress
ProteomicsMaximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies
Type I polyketide synthases (T1PKSs) hold an enormous potential as a rational production platform for the biosynthesis of speciality chemicals. However, despite the great progress in this field, the heterologous expression of PKSs remains a major challenge. One of the first measures to improve heter...
ORGANISM(S): Corynebacterium glutamicum Escherichia coli Pseudomonas putida KT2440 
2023-09-01 | PXD042749 | Pride
Codon optimization Heterologous expression Polyketide synthases 
TranscriptomicsDiscovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics
Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induc...
ORGANISM(S): Bifidobacterium breve UCC2003 
2011-12-16 | E-GEOD-31032 | biostudies-arrayexpress
TranscriptomicsThe heat shock response of killifish (Fundulus heteroclitus): candidate gene and heterologous microarray approaches.
Subspecies of the Atlantic killifish, Fundulus heteroclitus, differ in their maximum thermal tolerance. To determine whether there is a link between the heat shock response (HSR) and maximum thermal tolerance, we exposed 20ºC acclimated killifish from these subspecies to a 2hr heat shock at 34ºC ...
ORGANISM(S): Fundulus heteroclitus 
2010-06-20 | E-GEOD-18792 | biostudies-arrayexpress
GenomicsSystems-level engineering and characterization of Clostridium autoethanogenum through heterologous production of poly-3-hydroxybutyrate (PHB)
Systems-level engineering and characterization of Clostridium autoethanogenum through heterologous production of poly-3-hydroxybutyrate (PHB)
PRJNA476240 | ENA
UnknownHeterologous expression of cobalamin dependent class-III enzymes.
Not available
S-EPMC7585037 | biostudies-literature
GenomicsDeciphering Host Stress Responses for Predictable Heterologous Gene Expression in E. coli
Deciphering Host Stress Responses for Predictable Heterologous Gene Expression in E. coli
PRJNA1306061 | ENA
GenomicsAdaptive laboratory evolution with heterologous pgi
Adaptive laboratory evolution with heterologous pgi
PRJNA933234 | ENA
UnknownCurrent state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems.
Not available
S-EPMC10323146 | biostudies-literature
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