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ProteomicsIdentification of spidroin proteins in the silk glands of Nephila clavipes spiders
Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Spider silk  proteomics  liquid chromatography-tandem mass spectrometry  silk gut 
UnknownRac-GTPase promotes fibrotic TGF-β1 signaling and chronic kidney disease via EGFR, p53, and Hippo/YAP/TAZ pathways.
Not available
S-EPMC6704447 | biostudies-literature
MetabolomicsLongitudinal metabolomics and lipidomics analyses reveal markers of envenoming by Bothrops asper and Daboia russelii in an experimental murine model

Longitudinal metabolomics and lipidomics analysis was carried out in the blood plasma of mice injected intramuscularly with venoms of the viperid species Bothrops asper and Daboia russelii. Blood samples were collected 1, 3, 6 and 24 h after venom injection, and a control group ...

2022-09-21 | MTBLS5430 | MetaboLights
MetabolomicsDietary restriction of cysteine and methionine sensitizes gliomas to ferroptosis and induces alterations in energetic metabolism

Ferroptosis is mediated by lipid peroxidation of phospholipids containing polyunsaturated fatty acyl moieties. Glutathione, the key cellular antioxidant capable of inhibiting lipid peroxidation via the activity of the enzyme glutathione peroxidase 4 (GPX-4), is generated directly from the sulfur-...

2023-01-11 | MTBLS5959 | MetaboLights
MetabolomicsMetabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants
The differences in pungency of 'sirih' imply the probable occurrence of several variants of Piper betle L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected M...
2022-03-21 | MTBLS2585 | MetaboLights
MetabolomicsTargeting the SREBP-dependent lipidomic reprogramming by virus infection for broad-spectrum therapeutic intervention
Viruses are obligate intracellular parasites that exploit the host metabolic machineries to meet their extraordinary biosynthetic demands. Integrative transcriptomic and lipidomic profiling confirmed that Middle East respiratory syndrome coronavirus (MERS-CoV) infection reprograms the host lipid met...
2018-11-26 | MTBLS762 | MetaboLights
UnknownDNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species.
Not available
S-EPMC9935246 | biostudies-literature
UnknownAn organophosphonate strategy for functionalizing silicon photonic biosensors.
Not available
S-EPMC3291497 | biostudies-literature
UnknownThe Smarter Safer Homes Solution to Support Older People Living in Their Own Homes Through Enhanced Care Models: Protocol for a Stratified Randomized Controlled Trial.
Not available
S-EPMC8822419 | biostudies-literature
UnknownClinical and Economic Outcomes in Patients With Alpha-1 Antitrypsin Deficiency in a US Medicare Advantage Population.
Not available
S-EPMC11846658 | biostudies-literature
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