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ProteomicsExploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway
Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the l...
ORGANISM(S): Escherichia Coli 
2019-10-29 | PXD012697 | panorama
ProteomicsAn automated ‘cells-to-peptides’ sample preparation workflow for high-throughput, quantitative proteomic assays of microbes
Mass spectrometry–based quantitative proteomic analysis has proven valuable for clinical and biotechnology related research and development. Driving this value have been improvements in the sensitivity, resolution, and robustness of mass analyzers. However, manual sample preparation protocols are of...
ORGANISM(S): Escherichia Coli Rhodotorula Toruloides Pseudomonas Putida Kt2440 Saccharomyces Cerevisiae 
2019-08-23 | PXD014182 | panorama
ProteomicsLessons from Two Design-Build-Test-Learn Cycles of Dodecanol Production in Escherichia coli Aided by Machine Learning
The Design–Build–Test–Learn (DBTL) cycle, facilitated by exponentially improving capabilities in synthetic biology, is an increasingly adopted metabolic engineering framework that represents a more systematic and efficient approach to strain development than historical efforts in biofuels and bio-ba...
ORGANISM(S): Escherichia Coli 
2019-05-16 | PXD012312 | panorama
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