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GenomicsGenome-wide analysis reveals that budding yeast Rif1 binds to replication origins and at blocked replication forks to protect nascent DNA
This experiment details ChIP sequencing to decipher the binding sites of the Rif1 protein in budding yeast. Rif1 binds most strongly to telomeres where its binding is mediated by Rap1. To reduce telomere binding and help reveal Rap1-independent binding sites, a truncation mutant of Rif1 lacking the ...
ORGANISM(S): Saccharomyces cerevisiae 
2018-12-31 | E-MTAB-6736 | biostudies-arrayexpress
GenomicsSingle-cell replication timing analysis of hTERT-RPE1 cells depleted for SAF-A protein
The impact of depleting SAF-A (HNRNPU) on the genome-wide replication timing program in human hTERT-RPE1 cells was assessed by a single-cell replication timing analysis.
ORGANISM(S): Homo sapiens 
2021-12-15 | E-MTAB-10234 | biostudies-arrayexpress
GenomicsChIP-Seq analysis of Myc-tagged budding yeast Rif1
Genomic locations of Myc-tagged budding yeast Rif1 (including wilt-type and designer mutants) were analysed by ChIP-Seq. Mutants tested were rif1-7A and rif1-7E, in which Ser/Thr residues in the cluster of SQ/TQ sites were mutated to Ala or Glu, respectively. The performance of RIF1-9V5 was also tes...
ORGANISM(S): Saccharomyces cerevisiae 
2023-11-19 | E-MTAB-13451 | biostudies-arrayexpress
GenomicsChIP-Seq analysis of V5-tagged budding yeast Rif1
Genomic locations of V5-tagged budding yeast Rif1 (including wilt-type and designer mutants) were analysed by ChIP-Seq. Mutants tested were rif1-7A and rif1-7E, in which Ser/Thr residues in the cluster of SQ/TQ sites were mutated to Ala or Glu, respectively. We also tested tested rif1-∆594, in which...
ORGANISM(S): Saccharomyces cerevisiae 
2023-11-19 | E-MTAB-13452 | biostudies-arrayexpress
ProteomicsMass spectrometry analysis of the impact of replication stress upon phosphorylation and protein interaction of human RIF1 protein
Wild-type or S2265A version of GFP-RIF1 protein (isoform 1) was over-expressed in Flp-In-T-REx cells (Watts et al. 2020. eLife 9:e58020) and immunopurified using GFP-Trap magnetic agarose beads. Proteins were subjected to on-beads trypsin digestion and resulting peptides were analysed by mass spect...
ORGANISM(S): Homo sapiens (Human) 
2022-04-14 | PXD032015 | Pride
Rif1 Protein phosphorylation Replication stress 
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