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TranscriptomicsRif1 regulates initiation timing of late replication origins throughout the S. cerevisiae genome [ChIP-Seq]
Chromosomal DNA replication involves the coordinated activity of hundreds to thousands of replication origins. Individual replication origins are subject to epigenetic regulation of their activity during S-phase, resulting in differential efficiencies and timings of replication initiation during S-...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-01 | E-GEOD-55155 | biostudies-arrayexpress
GenomicsGenome-wide analysis reveals that budding yeast Rif1 binds to replication origins and at blocked replication forks to protect nascent DNA
This experiment details ChIP sequencing to decipher the binding sites of the Rif1 protein in budding yeast. Rif1 binds most strongly to telomeres where its binding is mediated by Rap1. To reduce telomere binding and help reveal Rap1-independent binding sites, a truncation mutant of Rif1 lacking the ...
ORGANISM(S): Saccharomyces cerevisiae 
2018-12-31 | E-MTAB-6736 | biostudies-arrayexpress
TranscriptomicsRif1 regulates initiation timing of late replication origins throughout the S. cerevisiae genome [array]
Chromosomal DNA replication involves the coordinated activity of hundreds to thousands of replication origins. Individual replication origins are subject to epigenetic regulation of their activity during S-phase, resulting in differential efficiencies and timings of replication initiation during S-...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-01 | E-GEOD-55130 | biostudies-arrayexpress
GenomicsRif1 Prevents Resection of DNA Breaks and Promotes Immunoglobulin Class Switching
DNA double-strand breaks (DSBs) represent a threat to the genome because they can lead to loss of genetic information and chromosome rearrangements. The DNA repair protein p53 binding protein 1 (53BP1) protects the genome by limiting nucleolytic processing of DSBs by a mechanism that requires its ph...
ORGANISM(S): Mus musculus 
2013-03-01 | E-GEOD-42298 | biostudies-arrayexpress
GenomicsHelicobacter pylori Rif1
Helicobacter pylori Rif1 Genome sequencing
PRJNA175544 | ENA
GenomicsGenome-wide distribution of histones modifications in mouse ESC Rif1+/+ and Rif1-/-
Analyzed histones modifications: H3K9 H3K4 H3K27 H4K20
ORGANISM(S): Mus musculus 
2015-12-24 | E-MTAB-3743 | biostudies-arrayexpress
ProteomicsASF1 acts with RIF1 to promote DNA end-joining in BRCA1 deficient cells
RIF1 acts downstream of 53BP1 to coordinate DNA double strand break repair pathway choice between non-homologous end joining (NHEJ) and homologous recombination (HR). Here we identified ASF1 as an endogenous RIF1-associated protein. We showed that ASF1 forms complex with RIF1 and regulates RIF1-depe...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD027727 | Pride
Rif1 Tap-ms Asf1 Interactome 
ProteomicsMass spectrometry analysis of the impact of replication stress upon phosphorylation and protein interaction of human RIF1 protein
Wild-type or S2265A version of GFP-RIF1 protein (isoform 1) was over-expressed in Flp-In-T-REx cells (Watts et al. 2020. eLife 9:e58020) and immunopurified using GFP-Trap magnetic agarose beads. Proteins were subjected to on-beads trypsin digestion and resulting peptides were analysed by mass spect...
ORGANISM(S): Homo sapiens (Human) 
2022-04-14 | PXD032015 | Pride
Rif1 Protein phosphorylation Replication stress 
GenomicsGenome-wide occupancy of Rif1FH in mouse ESC
Mammalian Rif1 defines the architecture of replication-timing domains interactions through the three-dimensional organization of the nuclear volume. Deletion of RIf1 in mammalian cells causes an initial alteration of three-dimensional chromatin organization which impacts first on replication timing ...
ORGANISM(S): Mus musculus 
2015-12-24 | E-MTAB-3502 | biostudies-arrayexpress
TranscriptomicsThe role of Rif1 in ES cells
Purpose:The goals of this study are to understand the mechanisms underlying reduced self-renewal and loss of pluripotency by depletion of Rif1. Methods: We performed global gene expression analysis of Rif1 knockdown ES cell lines using Affymetrix 430 2.0 arrays, compared to shRNA controls. J1 ES cel...
ORGANISM(S): Mus musculus 
2014-02-19 | E-GEOD-55129 | biostudies-arrayexpress
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