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ProteomicsTAP-GluN1, PSD95-TAP, WT (control) BNP-LC-MS/MS proteomics
TAP-GluN1 (840 kDa and 1.5 MDa), PSD95-TAP (1.5 MDa) and WT (control; 1.5 MDa) native complexes from Grin1^TAP/TAP, Dlg4^TAP/TAP, and WT mouse forebrain (cortex and hippocampus), respectively. Purified samples were seperated by blue native PAGE. Bands were excised, digested with trypsin. Peptides we...
ORGANISM(S): Mus musculus (Mouse) 
2016-04-20 | PXD000011 | Pride
Native complexes Synapse Nmdar 
Proteomics293T NME4 TAP-MS and TurboID-MS
To identify interacting proteins of NME4 in human cell lines, we using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T cells. We established stable cell line which expressing NME4 gene fused with SFB triple tags (S tag-2x Flag tag-SBP tag) or TurboID. We isolated, comb...
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD036624 | Pride
Tap-ms turboid-ms 
UnknownA straightforward Py-GC/MS methodology for quantification of microplastics in tap water.
Not available
S-EPMC11791246 | biostudies-literature
UnknownPlasma biomarkers TAP, CPA1, and CPA2 for the detection of pancreatic injury in rat: the development of a novel multiplex IA-LC-MS/MS assay and biomarker performance evaluation.
Not available
S-EPMC9968696 | biostudies-literature
ProteomicsInteractome of AMPK complex study using TAP-MS
The adenosine monophosphate-activated protein kinase (AMPK) complex is a key enzyme in regulating cellular energy homeostasis. This complex is well studied and it is known to function in many cellular pathways. However, the interactome of AMPK complex has not yet been systematically established, alt...
ORGANISM(S): Homo sapiens (Human) 
2020-01-09 | PXD015466 | Pride
Interactome Ampk Tap 
UnknownDisk solid-phase extraction of multi-class pharmaceutical residues in tap water and hospital wastewater, prior to ultra-performance liquid chromatographic-tandem mass spectrometry (UPLC-MS/MS) analyses.
Not available
S-EPMC9091434 | biostudies-literature
ProteomicsProtein interaction AP-MS data for TAP-CSAG1 and TAP-CSAG2 from HEK293 cells
Ten 15 cm2 plates of HEK293 cells stably expressing TAP-vector, TAP-CSAG1, or TAP-CSAG2 were lysed with TAP lysis buffer (10% (v/v) glycerol, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 2 mM EDTA, 0.1% (v/v) NP-40, 10 mM NaF, 0.25 mM Na3VO4, 50 mM β-glyerolphosphate, 2 mM DTT, and 1X protease inhibitor cock...
ORGANISM(S): Homo sapiens (Human) 
2020-09-01 | PXD020231 | Pride
Csag1 csag2 
ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-17 | MSV000083252 | MassIVE
ProteomicsSLX4 and SLX4IP TAP-MS - SLX4IP acts with SLX4 and XPF-ERCC1 to promote interstrand crosslink repair
Interstrand crosslinks (ICLs) are highly toxic DNA lesions, which are repaired via a complex process that requires the coordination of several DNA repair pathways. Defects in ICL repair result in Fanconi anemia (FA), which is diagnosed with bone marrow failure, development abnormalities and high inc...
ORGANISM(S): Homo sapiens (Human) 
2019-09-13 | PXD012521 | Pride
Sfb Slx4 Tap-ms Slx4ip 
ProteomicsTAP-MS of human Integrator subunits INTS10 and INTS13
Using a combination of proteomics, interaction studies and structural characterization we show that the INTS10-13-14 module of INT binds several transcriptional regulators and gene-specific transcription factors, whichcould form the molecular basis for a part of INT's gene specificity.
ORGANISM(S): Homo sapiens (Human) 
2024-06-27 | PXD041374 | Pride
Human Integrator Tap-ms 
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