Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap, instrument model
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Rene Frank
LAB HEAD: Seth Grant
PROVIDER: PXD000011 | Pride | 2016-04-20
REPOSITORIES: Pride
Frank René A W RA Komiyama Noboru H NH Ryan Tomás J TJ Zhu Fei F O'Dell Thomas J TJ Grant Seth G N SG
Nature communications 20160427
How neuronal proteomes self-organize is poorly understood because of their inherent molecular and cellular complexity. Here, focusing on mammalian synapses we use blue-native PAGE and 'gene-tagging' of GluN1 to report the first biochemical purification of endogenous NMDA receptors (NMDARs) directly from adult mouse brain. We show that NMDARs partition between two discrete populations of receptor complexes and ∼1.5 MDa supercomplexes. We tested the assembly mechanism with six mouse mutants, which ...[more]