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ProteomicsIdentifying the N-terminus of SLX4Nter in HeLa KO30 cells
The project was aimed at identifying the N-terminus of a truncated form of SLX4 (termed SLX4Nter) in HeLa KO30 cells. The HeLa KO30 cell line comes from a clone of HeLa Flp-In T-Rex cells (termed FITo) obtained through a CRISPR-Cas9 approach using commercially available plasmids from Santa Cruz Biot...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD029062 | Pride
Crispr-cas9 Slx4 N-terminus acetylation N-terminal truncation 
ProteomicsSearching for interactors of endogenous SLX4
The project was aimed at identifying binding partners of endogenous SLX4 in HeLa cells. Whole cell extracts from HeLa Flp-In T-Rex cells were used for immunoprecipitation performed with anti-SLX4 antibodies from Bethyl Laboratories (A302-270A and A302-269A), either used alone or in combination. To d...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD029084 | Pride
Endogenous slx4 Interactome Lc-msms 
ProteomicsSLX4 and SLX4IP TAP-MS - SLX4IP acts with SLX4 and XPF-ERCC1 to promote interstrand crosslink repair
Interstrand crosslinks (ICLs) are highly toxic DNA lesions, which are repaired via a complex process that requires the coordination of several DNA repair pathways. Defects in ICL repair result in Fanconi anemia (FA), which is diagnosed with bone marrow failure, development abnormalities and high inc...
ORGANISM(S): Homo sapiens (Human) 
2019-09-13 | PXD012521 | Pride
Sfb Slx4 Tap-ms Slx4ip 
ProteomicsSearching for new members of the SLX4 complex and for SLX4-complex members that are SUMOylated in vitro
The project was initially aimed at identifying new members of the SLX4 complex as well as members of the complex that are SUMOylated in vitro. The project was also aimed at assessing whether SUMOylation may change the compositon of the complex by reducing complex association of SUMOylated partners. ...
ORGANISM(S): Homo sapiens (Human) 
2020-03-05 | PXD012425 | Pride
Slx4 scaffold Protein sumoylation Interactome 
ProteomicsSearching for binding partners of the SLX4 577-795 region and assessing the impact of the SLX4 D614G and L618P cancer patient-derived mutations on their association with SLX4
The project was aimed at identifying new binding partners of SLX4 that associate with a region spanning the conserved amphipathic helix of SLX4 and the downstream BTB domain. It was also aimed at assessing which of those partners might have their association with SLX4 abrogated by the cancer patient...
ORGANISM(S): Homo sapiens (Human) 
2020-03-05 | PXD012426 | Pride
Slx4 scaffold Protein-protein binding domain Cancer associated mutations Interactome 
UnknownDimerization of SLX4 contributes to functioning of the SLX4-nuclease complex.
Not available
S-EPMC4889959 | biostudies-literature
Genomics
SLX4 sequencing
PRJNA611917 | ENA
UnknownSLX4 dampens MutSα-dependent mismatch repair.
Not available
S-EPMC8934664 | biostudies-literature
ProteomicsHA-mSLX4 wild type and SIMdependent interactors
SUMOylation is known to play important roles in the DNA damage response, however, a role for SUMOylation in interstrand crosslink repair remains enigmatic. We report on the regulation of the SLX4 nuclease scaffold protein by SUMOylation. SLX4 contains three SUMO-Interaction Motifs (SIMs). Mutating ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2015-03-02 | PXD001681 | Pride
Dna repair Slx4 Fanconi anemia Sumo Ubiquitin 
UnknownAssessment of SLX4 Mutations in Hereditary Breast Cancers.
Not available
S-EPMC3694110 | biostudies-literature
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