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The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMPactivated protein kinase (AMPK)-regulated endosomal sorting complex required for transport (ESCRT)-d...
ORGANISM(S): Saccharomyces Cerevisiae 
2022-02-06 | PXD031469 |
This project compares the novel ubiquitin affinity reagent OtUBD with a monoclonal ubiquitin antibody FK2 in their abilities to facilitae the profiling of the ubiquitylated proteome of HeLa cells. Enrichments were done side by side using a semi-denaturing condition.
ORGANISM(S): Homo sapiens (Human) 
2022-05-30 | PXD032675 | Pride
This project includes two separate experiments where the ubiquitylated proteome of the budding yeast Saccharomyces cerevisiae was enriched from whole cell lysate by affinity purification using a novel OtUBD affinity resin. In the first experiment, two distinct experimental conditions (native, denatu...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-05-30 | PXD032294 | Pride
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