Here we reported 226 sperm proteins from the Hong Kong oyster Crassostrea hongkongensis. Proteins extracted from three sperm samples were separated by SDS-PAGE, analyzed by LC-MS/MS and identified using Mascot.
Proteins were extracted from the perivitelline fluid of the apple snail Pomacea maculata eggs. And then proteins were seperated on SDS-PAGE gel and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.
