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ProteomicsScreening for novel immungenic proteins of Klebsiella pneumoniae MGH 78578
The screening of a cDNA derived expression library of Klebsiella pneumoniae MGH 78578 expressed in E.coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (ompA, mdh) and negative (pyrC, gapA) refer...
ORGANISM(S): Klebsiella pneumoniae 
2014-02-02 | E-GEOD-52536 | biostudies-arrayexpress
ProteomicsVerfication of immunogenic nature by microarray screening
Screening of 14 novel proteins derived from Klebsiella pneumoniae MGH 78578 identified prior via screening of cDNA libraries. The full-length proteins were attached using a specific HaloTag to their corresponding ligand surface, HaloLink. Screening was performed using two different polyclonal antibo...
ORGANISM(S): Klebsiella pneumoniae 
2014-02-02 | E-GEOD-52538 | biostudies-arrayexpress
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