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For the purpose of analyzing mechanisms related to the cis-diamminedichloroplatinum (CDDP) resistance in head and neck squamous cell carcinoma (head and neck SCC), we employed a nasal squamous cell carcinoma (nasal SCC) cell line RPMI2650 and its CDDP resistant substrain RPMI2650CR previously descri...
ORGANISM(S): Homo sapiens 
We examined the microarray-based expression profiles of the cells to identify genes associated with gemcitabine resistance Using three set of parental and resistant cell lines, PK-9 and RPK-9, PK-1 and RPK-1, and PK-59 and RPK-59, we studied mRNA transcription levels by microarray analysis to see if...
ORGANISM(S): Homo sapiens 
We performed microarray analyses before and after induction of S100A4 using PCI-35 and PCI-43, and identified 72 and 18 genes that were two fold or more commonly upregulated or downregulated, respectively, in these two cell lines. Using two cell lines, PCI-35 and PCI-43, transfected with S1004A expr...
ORGANISM(S): Homo sapiens 
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
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