Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression signature of gemcitabine resistance in human pancreatic cancer cell lines

ABSTRACT: We examined the microarray-based expression profiles of the cells to identify genes associated with gemcitabine resistance Using three set of parental and resistant cell lines, PK-9 and RPK-9, PK-1 and RPK-1, and PK-59 and RPK-59, we studied mRNA transcription levels by microarray analysis to see if there are any of the key molecules for development of acquisition of the GEM resistance.

ORGANISM(S): Homo sapiens

SUBMITTER: yuriko saiki

PROVIDER: E-GEOD-35141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells.

Saiki Yuriko Y Yoshino Yuki Y Fujimura Hiroko H Manabe Tatsuya T Kudo Yuki Y Shimada Miki M Mano Nariyasu N Nakano Tomohiro T Lee Yoonha Y Shimizu Shinjiro S Oba Shinya S Fujiwara Sho S Shimizu Hideyuki H Chen Na N Nezhad Zhaleh Kashkouli ZK Jin Guo G Fukushige Shinichi S Sunamura Makoto M Ishida Masaharu M Motoi Fuyuhiko F Egawa Shinichi S Unno Michiaki M Horii Akira A

Biochemical and biophysical research communications 20120403 1

Although gemcitabine is the most effective chemotherapeutic agent against pancreatic cancer, a growing concern is that a substantial number of patients acquire gemcitabine chemoresistance. To elucidate the mechanisms of acquisition of gemcitabine resistance, we developed gemcitabine-resistant cell lines from six human cancer cell lines; three pancreatic, one gastric, one colon, and one bile duct cancer. We first analyzed gemcitabine uptake using three paired parental and gemcitabine resistant pa ...[more]

PMID: 22490663

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Project description:In this study, we demonstrate that insulin is produced not only in the mammalian pancreas but also in adult neuronal cells derived from hippocampus and olfactory bulb. Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampus and olfactory bulb-derived neural stem cells. Our analysis indicates that the balance between Wnt3, which triggers the expression of insulin via NeuroD1 transcription factor, and IGFBP-4, which inhibits the original Wnt3 action, is regulated depending on the diabetic status. We also show that adult neural progenitors derived from diabetic animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of diabetic animals reduces glucose levels. This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes. In this study, we demonstrate that insulin is produced not only in the mammalian pancreas but also in adult neuronal cells derived from hippocampus and olfactory bulb. Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampus and olfactory bulb-derived neural stem cells. Our analysis indicates that the balance between Wnt3, which triggers the expression of insulin via NeuroD1 transcription factor, and IGFBP-4, which inhibits the original Wnt3 action, is regulated depending on the diabetic status. We also show that adult neural progenitors derived from diabetic animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of diabetic animals reduces glucose levels. This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes. Total four different samples, gene expressions in hippocampal derived neural stem cells (HPC NSC), that in Olfactory bulb-derived neural stem cells (OB NSC), that in neurons derived from the HPC NSCs (HPC Neu) and that in neurons derived from the OB NSCs (OB Neu) were independently analyzed. Three independent experiments were performed to prepare each cell sample, and the extracted total RNAs from each cell source were mixed to apply following microarray analysis (Four independent RNA sample; HPC NSC, OB NSC, HPC Neu and OB Neu).

Dataset Information

Gene expression signature of gemcitabine resistance in human pancreatic cancer cell lines

Publications

DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells.

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