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TranscriptomicsWide-spread disruption of transcription termination in HSV-1 infection: Next generation sequencing of total and newly transcribed (4sU-RNA) RNA
Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. N...
ORGANISM(S): Homo sapiens 
2015-05-26 | E-GEOD-59717 | biostudies-arrayexpress
TranscriptomicsWide-spread disruption of transcription termination in HSV-1 infection: Next-generation sequencing of translational activityd by ribosome profiling
Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at various times during infection with minor modification to the protocol described in Stern-Ginossar N et al., Science...
ORGANISM(S): Homo sapiens 
2015-05-26 | E-GEOD-60040 | biostudies-arrayexpress
TranscriptomicsHuman cellular models for Herpes Simplex Virus, type 1 (HSV-1) using induced pluripotent stem cell derived neuronal lineages
Herpes simplex virus type 1 (HSV-1) is a 152 Kb double stranded DNA alpha-herpesvirus, which establishes long life latent infection in sensory neurons. Most of our knowledge regarding HSV-1 latency comes from in vivo studies using small animal models, mainly rodents and rabbits, which are not natura...
ORGANISM(S): Homo sapiens 
2014-05-02 | E-GEOD-46041 | biostudies-arrayexpress
TranscriptomicsInduced pluripotent stem cell derived neuronallineages as human cellular models for Herpes Simplex Virus, type 1 (HSV-1) infections
Herpes simplex virus type 1 (HSV-1) is a 152 Kb double stranded DNA alpha-herpesvirus, which establishes long life latent infection in sensory neurons. Most of our knowledge regarding HSV-1 latency comes from in vivo studies using small animal models, mainly rodents and rabbits, which are not natura...
ORGANISM(S): Homo sapiens 
2014-05-02 | E-GEOD-46042 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of HSV-1 infected murine embryonic stem cells
Transcription profiling of HSV-1 infected murine embryonic stem cells
ORGANISM(S): Mus musculus 
2012-01-02 | E-MTAB-882 | biostudies-arrayexpress
TranscriptomicsA novel miRNA produced during lytic HSV-1 infection is important for efficient replication in tissue culture.
We report a HSV-1 encoded miRNA present during lytic infection that influences replication efficiency in a tissue culture model. miRNAs were identified using high-throughput sequencing of HSV-1 infected epithelial cells. Functional assays were performed by mutating the miRNA coding region and test...
ORGANISM(S): Homo sapiens 
2012-05-08 | E-GEOD-37726 | biostudies-arrayexpress
TranscriptomicsImpaired intrinsic immunity to HSV-1 in human iPSC-derived TLR3-deficient CNS cells
In the course of primary infection with herpes simplex virus 1 (HSV-1), children with inborn errors of the TLR3- and UNC-93B-dependent induction of IFN-α/β and/or IFN-λ immunity are prone to HSV-1 encephalitis (HSE) 1-3. The cells responsible for HSE in these children have yet to be identified. We t...
ORGANISM(S): Homo sapiens 
2012-11-29 | E-GEOD-40593 | biostudies-arrayexpress
MetabolomicsBroad Spectrum MS analysis of mouse microglia cells from Anxiety Prone HSV-Latently Infected Obese Mice
Not available
ORGANISM(S): Mus musculus 
ST000359 | MetabolomicsWorkbench
TranscriptomicsSequencing of HSV-1 genomic DNA to identify genomic variation resulting from selective pressure imposed by mutation of the PP1 binding site in the pUL21 gene
The pUL21 gene is essential for efficient replication and dissemination in herpes simplex virus 1 (HSV-1) but the molecular basis for its function is not well understood. Experimental work revealed that pUL21 is a virus-encoded adaptor of protein phosphatase 1 (PP1). Mutation of the specific motif i...
ORGANISM(S): Human alphaherpesvirus 1 
2021-07-31 | E-MTAB-10788 | biostudies-arrayexpress
MetabolomicsBroad Spectrum MS analysis of mouse peripheral blood mononuclear cells (PBMC) from Anxiety Prone HSV-Latently Infected Obese Mice
Not available
ORGANISM(S): Mus musculus 
ST000363 | MetabolomicsWorkbench
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