The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells, in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient salvage remain elusive. Numerous ultrastructural studies have documented a plasma membrane invagination with a d...
