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ProteomicsIdenfication the receptor on KRAS p.G12D cell that interact with FN1859
To capture the unknown receptors, we purified recombinant His-tagged FN1859 proteins from Escherichia coli and performed His-tag pull-down assays with WT, KRAS p.G13D and KRAS p.G12D cell lysates. Then the bands submitted for mass spectrometry (MS) protein identification.
ORGANISM(S): Homo sapiens (Human) 
2024-01-25 | PXD048684 | Pride
Kras p.g12d fn1859 
ProteomicsIdentification of the cellular component interacted with F. nucleatum.
We incubated F. nucleatum lysates with biotinylated proteins from KRAS p.G12D, KRAS p.G13D and KRAS WT cells respectively. Subsequent western blot analysis showed that three candidate proteins from F. nucleatum might interact with KRAS p.G12D cells. The bands of interest were then submitted for mass...
ORGANISM(S): Fusobacterium nucleatum CTI-1 
2024-01-25 | PXD048686 | Pride
Kras g12d fn1859 
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