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TranscriptomicsIn vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs
Dicer is an RNase III-family endoribonuclease and haploinsufficient tumor suppressor that is required for the biogenesis of miRNAs, yet in vivo structure-function characterization of its RNase IIIA and IIIB domains have not been reported. In murine Dicer knockout fibroblasts, we expressed human Dic...
ORGANISM(S): Mus musculus 
2012-07-13 | E-GEOD-36978 | biostudies-arrayexpress
TranscriptomicsTranscriptome wide identification of Dicer binding in human and C. elegans reveals a variety of substrates (CEL RNA-Seq)
Dicer is a deeply conserved endoribonuclease with key functions in small RNA biogenesis. Here we employed PAR-CLIP/iPAR-CLIP to identify direct Dicer binding sites in the transcriptomes of human cells and C.elegans. We found hundreds of novel miRNAs and non-canonical Dicer substrates with high sensi...
ORGANISM(S): Caenorhabditis elegans 
2014-11-20 | E-GEOD-55329 | biostudies-arrayexpress
TranscriptomicsA variety of Dicer substrates in human and C. elegans (CEL RNA-seq)
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer binding site...
ORGANISM(S): Caenorhabditis elegans 
2014-11-20 | E-GEOD-63435 | biostudies-arrayexpress
TranscriptomicsTranscriptome wide identification of Dicer binding in human and C. elegans reveals a variety of substrates (HEK RNA-Seq)
Dicer is a deeply conserved endoribonuclease with key functions in small RNA biogenesis. Here we employed PAR-CLIP/iPAR-CLIP to identify direct Dicer binding sites in the transcriptomes of human cells and human. We found hundreds of novel miRNAs and non-canonical Dicer substrates with high sensitivi...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-55326 | biostudies-arrayexpress
TranscriptomicsHuman nuclear Dicer restricts the deleterious accumulation of endogenous double strand RNA
Dicer is a central enzymatic player in RNA interference (RNAi) pathways that acts to regulate gene expression in nearly all eukaryotes. Although the cytoplasmic function of Dicer is well documented in mammals, there is little known about any possible nuclear function. Here we show that Dicer is pre...
ORGANISM(S): Homo sapiens 
2014-04-10 | E-GEOD-56648 | biostudies-arrayexpress
TranscriptomicsA variety of Dicer substrates in human and C. elegans (HEK RNA-seq)
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer binding site...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-63475 | biostudies-arrayexpress
TranscriptomicsA variety of Dicer substrates in human and C. elegans (HEK PAR-CLIP)
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer binding site...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-63437 | biostudies-arrayexpress
TranscriptomicsA variety of Dicer substrates in human and C. elegans (HEK small RNA)
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer binding site...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-63458 | biostudies-arrayexpress
Proteomics4-HNE modification of human recombinant Dicer
We used mass spectrometry analysis (nanoLC-MS/MS) to map 4-HNE-modified residues of human Dicer exposed in vitro to 4-HNE in a concentration-dependent maner
ORGANISM(S): Homo sapiens (Human) 
2023-11-17 | PXD034113 | Pride
Human dicer 4-hne Lc-ms/ms 
TranscriptomicsTranscriptome wide identification of Dicer binding in human and C. elegans reveals a variety of substrates (HEK PAR-CLIP)
Dicer is a deeply conserved endoribonuclease with key functions in small RNA biogenesis. Here we employed PAR-CLIP/iPAR-CLIP to identify direct Dicer binding sites in the transcriptomes of human cells and human. We found hundreds of novel miRNAs and non-canonical Dicer substrates with high sensitivi...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-55324 | biostudies-arrayexpress
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