Human serum binding

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Transcriptomics (8021)
Unknown (3841)
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Organisms

Homo sapiens (5247)
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synthetic construct (7)
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Organisms

Homo sapiens (126)
Mus musculus (59)
synthetic construct (3)
human gut metagenome (2)
Helicobacter pylori PMSS1 (1)
Human immunodeficiency virus (1)
Macaca mulatta (1)
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Klebsiella pneumoniae (1)
Candida albicans (1)
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Rattus norvegicus (1)

Repository

biostudies-arrayexpress (8830)
pride (435)
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geo (77)
jPOST (37)
iProX (31)
PanoramaPublic (19)
PeptideAtlas (5)
GPMDB (4)
dbGaP (2)
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PAXDB (2)
GNPS (1)
LINCS (1)

Tissue

Cell culture (112)
Blood serum (68)
Epithelial cell (43)
Blood plasma (37)
Permanent cell line cell (25)
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Blood (15)
B cell (13)
Skin (13)
Brain (10)
T cell (10)
Endothelial cell of umbilical vein (9)
Hepatocyte (9)
Blood cell (8)
Umbilical vein (8)
Cell suspension culture (7)
Colon (7)
Early embryonic cell (6)
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Lung (6)
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Liver (5)
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None (2)
Prostate carcinoma (2)
Carcinoma (2)
Multiple myeloma (2)
Neuroendocrine tumor (1)
XCGD (1)
With bone lytic lesion (1)
Familial combined hyperlipidemia (1)
Testicular seminoma (1)

Technology Type

Mass Spectrometry (435)
Shotgun proteomics (231)
Mass spectrometry (153)
Mass spectrometry assay (80)
Bottom-up proteomics (72)
Affinity purification coupled with mass spectrometry proteomics (71)
Data-dependent acquisition (44)
Data-independent acquisition (31)
Gel-based experiment (26)
NMR spectroscopy (24)
SWATH MS (21)
Chemical cross-linking coupled with mass spectrometry proteomics (15)
Top-down proteomics (11)
SRM/MRM (10)
Affinity proteomics (5)
NMR spectroscopy assay (4)
Immunopeptidomics (4)
All-ion fragmentation (3)
Illumina HiSeq 2500, ILLUMINA (2)
ILLUMINA, Illumina HiSeq 2500 (2)
Illumina HiSeq 2500 (2)
Illumina CytoSNP, Illumina HumanOmni2.5, ILLUMINA, Illumina 450k, Illumina HiSeq 2000 (1)
MeDIP-seq (1)
ILLUMINA, Illumina HiSeq 2000 (1)
Illumina SNP array, Illumina HiSeq 2000, Agilent CGH array (1)
Illumina HiSeq 2000, Illumina Genome Analyzer II, ILLUMINA (1)
Illumina HiSeq 2000 (1)
Unknown (1)
Fluorescence intensity (1)
Microwestern (1)

Instrument Platform

Illumina NovaSeq 6000 (210)
NextSeq 500 (109)
Illumina HiSeq 2500 (96)
Illumina HiSeq 4000 (89)
Illumina HiSeq 2000 (83)
NextSeq 550 (33)
NextSeq 2000 (24)
Illumina NovaSeq X (21)
Illumina MiSeq (20)
Illumina HiSeq 1500 (19)
Illumina Genome Analyzer IIx (13)
Illumina Genome Analyzer II (11)
GenePix 4000B [Axon Instruments] (10)
Illumina HiSeq 3000 (9)
Element AVITI (7)
G2565AA DNA microarray scanner [Agilent] (7)
G2565BA DNA microarray scanner [Agilent] (6)
Illumina HiSeq 1000 (6)
418 [Affymetrix] (5)
DNBSEQ-G400 (5)
Agilent High resolution C scanner (4)
G2500A Hewlett Packard (4)
Axon- GenePix4000B (4)
Ion Torrent Proton (4)
PromethION (3)
Axon GenePix4000B (3)
None (3)
ScanArray [PerkinElmer] (3)
Affymetrix Fluidics Station 400 (3)
Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)

Publication Date

2015 (1182)
2013 (1166)
2010 (1082)
2014 (1056)
2012 (903)
2011 (846)
2016 (554)
2008 (468)
2009 (374)
2025 (270)
2026 (245)
2007 (225)
2022 (203)
2023 (199)
2024 (184)
2021 (177)
2018 (126)
2020 (123)
2017 (107)
2019 (100)
2005 (89)
2006 (87)
2004 (15)
2003 (12)
2002 (4)
2027 (1)

UNIPROT ID/AC

5833.MAL13P1.267 (2)
5833.MAL13P1.269 (2)
5833.MAL13P1.325 (2)
5833.MAL13P1.334 (2)
5833.MAL7P1.78 (2)
5833.MAL8P1.208 (2)
5833.MAL8P1.320 (2)
5833.PF11_0033 (2)
5833.PF11_0263 (2)
5833.PF11_0512 (2)
5833.PF13_0305 (2)
5833.PF13_0321 (2)
5833.PF14_0001 (2)
5833.PF14_0009 (2)
5833.PF14_0173 (2)
5833.PF14_0734 (2)
5833.PF14_0751 (2)
5833.PF14_0756 (2)
5833.PF14_0759 (2)
5833.PFA0105w (2)
5833.PFA0120c (2)
5833.PFA0705c (2)
5833.PFB0050c (2)
5833.PFB0280w (2)
5833.PFB0925w (2)
5833.PFB0946c (2)
5833.PFC1011c (2)
5833.PFC1075w (2)
5833.PFD0075w (2)
5833.PFD0100c (2)

ENSEMBL ID

ENSECAG00000000548 (3)
ENSECAG00000001810 (3)
ENSECAG00000004201 (3)
ENSECAG00000004903 (3)
ENSECAG00000004960 (3)
ENSECAG00000004973 (3)
ENSECAG00000006095 (3)
ENSECAG00000007714 (3)
ENSECAG00000008545 (3)
ENSECAG00000008615 (3)
ENSECAG00000008739 (3)
ENSECAG00000009069 (3)
ENSECAG00000009556 (3)
ENSECAG00000009752 (3)
ENSECAG00000010481 (3)
ENSECAG00000010619 (3)
ENSECAG00000010913 (3)
ENSECAG00000010963 (3)
ENSECAG00000011256 (3)
ENSECAG00000011847 (3)
ENSECAG00000011854 (3)
ENSECAG00000012299 (3)
ENSECAG00000012362 (3)
ENSECAG00000013486 (3)
ENSECAG00000014224 (3)
ENSECAG00000014414 (3)
ENSECAG00000014858 (3)
ENSECAG00000015505 (3)
ENSECAG00000015875 (3)
ENSECAG00000016475 (3)

CHEBI ID

CHEBI:16919 (58)
CHEBI:30769 (58)
CHEBI:16737 (57)
CHEBI:15428 (50)
CHEBI:15891 (46)
CHEBI:15354 (43)
CHEBI:16704 (41)
CHEBI:30915 (40)
CHEBI:28044 (39)
CHEBI:18257 (39)
CHEBI:17596 (39)
CHEBI:16449 (38)
CHEBI:27897 (37)
CHEBI:17368 (37)
CHEBI:25017 (36)
CHEBI:27266 (36)
CHEBI:17295 (36)
CHEBI:18050 (35)
CHEBI:17268 (35)
CHEBI:16015 (35)
CHEBI:18089 (35)
CHEBI:26271 (34)
CHEBI:18186 (34)
CHEBI:28300 (34)
CHEBI:25094 (34)
CHEBI:18237 (34)
CHEBI:17154 (34)
CHEBI:15756 (34)
CHEBI:16811 (33)
CHEBI:24898 (33)

Metabolite Name

Taurine (54)
Creatine (49)
Uridine (46)
Creatinine (45)
Hypoxanthine (44)
Inosine (42)
L-Glutamine (41)
L-Tyrosine (41)
L-Histidine (40)
Xanthine (40)
Choline (39)
Uric acid (39)
Guanosine (39)
L-Phenylalanine (39)
L-Tryptophan (37)
Adenosine (36)
L-Valine (36)
Hippuric acid (36)
Uracil (34)
Betaine (34)
L-Proline (34)
Deoxycholic acid (33)
L-Arginine (33)
Citric acid (33)
Adenine (33)
Ornithine (32)
Glycine (31)
L-Alanine (31)
Cholic acid (30)
Guanine (30)

First Public Date

2014 (50)
2023 (32)
2025 (30)
2024 (29)
2026 (19)
2022 (10)
2020 (10)
2021 (9)
2016 (8)
2019 (7)
2018 (7)
2017 (5)
2015 (2)
2013 (2)

Classification

bacteria (2)
viruses (1)
fungi (1)

Modification

4x(15)N labeled L-arginine (6)
2x(15)N labeled L-lysine (4)
4-dinitrophenyl modified residue (1)

Study type

Transcription profiling by array (5503)
RNA-seq of coding RNA (1027)
ChIP-seq (638)
Methylation profiling by array (164)
Unknown experiment type (161)
RNA-seq of non coding RNA (147)
Other (137)
ChIP-chip by tiling array (129)
RNA-seq of coding RNA from single cells (123)
Comparative genomic hybridization by array (98)
Proteomic profiling by array (94)
Methylation profiling by high throughput sequencing (75)
ChIP-chip by array (68)
Transcription profiling by SAGE (41)
Cell line - High-throughput sequencing (40)
RNA-seq of total RNA (38)
MicroRNA profiling by array (27)
DNA-seq (27)
Transcription profiling by RT-PCR (26)
Genotyping by array (25)
RNAi profiling by array (23)
ATAC-seq (21)
Transcription profiling by tiling array (20)
CUT&RUN (15)
MicroRNA profiling by high throughput sequencing (11)
High-throughput sequencing (10)
Antigen profiling (9)
Human - High-throughput sequencing (8)
Animal - High-throughput sequencing (8)
Cell line - One-color microarray (7)

Release Date

2015 (1337)
2013 (1288)
2014 (1171)
2010 (1153)
2012 (1003)
2011 (957)
2016 (699)
2022 (532)
2008 (509)
2021 (456)
2009 (437)
2023 (420)
2020 (395)
2025 (375)
2024 (348)
2018 (299)
2019 (285)
2017 (274)
2007 (250)
2026 (152)
2005 (113)
2006 (109)
2004 (27)
2003 (25)
2000 (15)
2002 (14)
2001 (8)
1999 (8)
1998 (8)
1997 (3)

Lab affiliation

University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry (22)
Infection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, VIC 3800, Australia (4)
King's College London (4)
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (4)
Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 23 Uppsala, Sweden. (4)
1VIB-UGent Center for Medical Biotechnology, VIB, 9052 Ghent, Belgium 2Department of Biomolecular Medicine, Ghent University, 9052 Ghent, Belgium (2)
Analytical Chemistry, Department of Chemistry – BMC and Science for Life Laboratory, Uppsala University, Box 599, 751 24 Uppsala, Sweden (2)
Boston University Medical Campus 670 Albany St., Rm. 509 Boston, MA 02118 USA (2)
CRUK - Beatson Institute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom (2)
Cancer Science Institute of Singapore (CSI), National University of Singapore (NUS) (2)
Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands (2)
Department of Biochemistry and Molecular Biology and the Biomedicine Discovery Institute; Monash University; Clayton 3800; Victoria; Australia (2)
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany (2)
Discovery Proteomics Facility Target Discovery Institute University of Oxford (2)
Eppley Institute University of Nebraska Medical Center (2)
IRCCS Istituto G Gaslini (2)
King's College London British Heart Foundation Centre, School of Cardiovascular Medicine and Sciences, King's College London, London, UK. (2)
Moffitt Cancer Center Tampa, FL, USA (2)
Novartis Institute of Biomedical Research, Cambridge, MA (2)
UCSF (2)
Vanderbilt University Proteomics Core (2)
Wayne State University Chemistry (2)
-Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht -Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht 3584 CH, Netherlands. -Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH, Netherlands. (1)
1 Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, the Netherlands 2 Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, the Netherlands (1)
1 VIB-UGent Center for Medical Biotechnology, B9052 Ghent, Belgium; 2 Department of Biomolecular Medicine, Ghent University, B9052 Ghent, Belgium. (1)
1. Nephrology Department of Guilin No. 924 Hospital, Guangxi Key laboratory of Metabolic Diseases Research, Guilin Key laboratory of Kidney Diseases Research, 541002, Guilin, Guangxi, People’s Republic of China. 2. Clinical Medical Research Center, the Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital), 518020, Shenzhen, Guangdong, People’s Republic of China. (1)
1. School of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia 2. Institute for Glyco-core Research (iGCORE), Nagoya University, Nagoya, Japan (1)
1. School of Natural Sciences, Macquarie University, Sydney, New South Wales, Australia 2. Institute for Glyco-core Research (iGCORE), Nagoya University, Nagoya, Japan (1)
1.Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA 92697, USA 2.Department of Ophthalmic Research, Cleveland Clinic, Cleveland, OH 44195, USA 3.Department of Physiology & Biophysics, School of Medicine, University of California-Irvine, Irvine, CA 92697, USA (1)
1Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (1)

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A Metabonomic Comparison of Human Serum and Plasma Subtypes Using Untargeted NMR Spectroscopy and UPLC-MS (NMR assays)

INTRODUCTION: Differences in the metabolite profiles between serum and plasma are incompletely understood. OBJECTIVES: To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes. METHODS: We analyzed serum, platelet rich plasma (PRP), platelet poo...

2018-07-25 | MTBLS470 | MetaboLights

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease

Dysregulated maternal fatty acid metabolism increases the risk of congenital heart disease (CHD) in offspring with an unknown mechanism, and the effect of folic acid fortification in preventing CHD is controversial. Using gas chromatography coupled to either a flame ionization detector or mass spect...

2023-03-24 | MTBLS6982 | MetaboLights

A Metabonomic Comparison of Human Serum and Plasma Subtypes Using Untargeted NMR Spectroscopy and UPLC-MS (UPLC-MS assays)

2018-07-25 | MTBLS465 | MetaboLights

Serum protein N-glycosylation changes in multiple myeloma

BACKGROUND: Multiple myeloma is characterized by clonal proliferation of malignant plasma cells in the bone marrow that produce monoclonal immunoglobulins. N-glycosylation changes of these monoclonal immunoglobulins have been reported in multiple myeloma, but previous studies on...

2022-04-27 | MTBLS676 | MetaboLights

targeted metabolomics of rat's serum

Podophyllotoxin (PPT) exhibits limited clinical utility due to its nephrotoxicity, and its underlying mechanisms remain poorly understood. This study employs the toxicological evidence chain (TEC) framework and integrated multi-omics analyses to investigate the potential involvement of the microbiot...

2026-06-27 | MTBLS14868 | MetaboLights

A Cross-Platform Metabolomics Comparison Identifies Serum Metabolite Signatures of Liver Fibrosis Progression in Chronic Hepatitis C Patients

Metabolomics offers new insights into disease mechanisms that is enhanced when adopting orthogonal instrumental platforms to expand metabolome coverage, while also reducing false discoveries by independent replication. Herein, we report the first inter-method comparison when using multisegment injec...

2023-08-22 | MTBLS3111 | MetaboLights

Chlorogenic acid improves obesity by regulating serum metabolites

Obesity, a globally prevalent chronic metabolic disorder, has been increasingly confirmed to involve gut microbiota as a pivotal regulator in its pathogenesis. Chlorogenic acid (CGA) has been reported to exert beneficial effects on lipid metabolism regulation and obesity amelioration. However, t...

2025-09-18 | MTBLS13009 | MetaboLights

Serum Metabolomic Profiling Reveals Load-Specific Adaptations to Resistance Training

This study deposits the untargeted ¹H-NMR serum metabolomics dataset of a parallel-group, longitudinal resistance training intervention designed to identify load-specific and training-status-specific metabolomic signatures in healthy young men. Seventeen healthy young men (age 24 ± 3 yr; height 173 ...

2026-07-20 | MTBLS15091 | MetaboLights

Comparative metabolomics reveals serum metabolites changes in goats during different developmental stages

Goats can provide meat, milk and skins for humans and are livestock with high economic benefits. However, despite their economic significance, the comprehensive analysis of goats' serum metabolic profile and its intricate alterations throughout their developmental journey remains conspicuously ab...

2024-06-04 | MTBLS8944 | MetaboLights

Large-scale non-targeted serum metabolomics in the Swedish Twin Registry

The TwinGene project, conducted between 2004 and 2008, is a population-based Swedish study of twins born between 1911 and 1958. The study participants have previously participated in a telephone interview called Screening Across the Lifespan Twin Study, conducted between 1998 and 2002. To be include...

2014-08-30 | MTBLS93 | MetaboLights