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To study the gene profiling of different cardiac adipogenic progenitor populations, we isolated individual cells from postnatal 3-week heart and performed single-cell RNA-sequencing. Single cell suspensions from isolated mouse heart were prepared by retrograde Langendorff perfusion. Briefly, mouse a...
ORGANISM(S): Mus musculus 
Fibroblastic reticular cells (FRC) shape the organization of secondary lymphoid organs and actively promote the induction of immune responses by coordinating the interaction of innate and adaptive immune cells. However, the mechanisms underlying FRC functions during viral infections have remained la...
ORGANISM(S): Mus musculus 
Basonuclin, which is a zinc-finger protein found in abundance only in the keratinocytes of the stratified epithelium, male germ cells and oocytes, qualifies as a maternal-effect gene because the source of pre-implantation embryonic basonuclin is maternal. Using a transgenic-RNAi approach, we knocke...
ORGANISM(S): Mus musculus 
In this study, we used proteomics analysis to confirm the immunoglobulin purification efficiency from human plasma samples. Protein components of plasma, supernatant fraction after affinity purification, and the on bead digestion fraction were performed.
ORGANISM(S): Homo sapiens (Human) 
2024-01-22 | PXD047040 | Pride
To identify bacterial transcripts that may be associated with type I IFN production in Salmonella enterica subsp typhimurium (SL1344) infected macrophages we transformed macrophages with an ISRE-GFP reporter construct and sorted separate populations of GFP positive and GFP negative infected macropha...
ORGANISM(S): Mus musculus 
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