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ProteomicsQuantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.
Extracellular vesicles (EVs) are lipid bilayered vesicles released into extracellular milieu by most of cells. Currently, various purification methods of EVs have been established but most of studies use ultracentrifugation-based purification of EVs. However, these methods have the problem of co-pur...
ORGANISM(S): Homo sapiens (Human) 
2021-08-30 | PXD001355 | Pride
Non-vesicular contaminants Quantitative proteomics Extracellular vesicles Trypsin treatment 
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-69 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters and used to valdiate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-68 | biostudies-arrayexpress
UnknownSubstance P blocks ovariectomy-induced bone loss by modulating inflammation and potentiating stem cell function.
Not available
S-EPMC7655156 | biostudies-literature
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3513 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part 3
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RN...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3507 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3516 | biostudies-arrayexpress
UnknownValidation of Enhancer Regions in Primary Human Neural Progenitor Cells using Capture STARR-seq.
Not available
S-EPMC10983874 | biostudies-literature
UnknownSingle-cell genomics and regulatory networks for 388 human brains.
Not available
S-EPMC10983939 | biostudies-literature
UnknownSingle-cell genomics and regulatory networks for 388 human brains.
Not available
S-EPMC11365579 | biostudies-literature
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