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ProteomicsZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple an...
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD018273 | Pride
Affinity enrichment Moac Imac Mass spectrometry Protein phosphorylation Functional proteomics Cell signalling Magnetic particles 
ProteomicsFe-IMAC column based phospho enrichment
Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation
ORGANISM(S): Homo sapiens (Human) 
2015-08-19 | PXD001060 | Pride
Hsax Tio2 Ti-imac Ptm Phospho Fe-imac 
ProteomicsDefeating major contaminants in Fe3+-IMAC phosphopeptide enrichment
Optimized sample preparation workflow for Fe-IMAC phosphopeptide enrichment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-02-19 | PXD008289 | Pride
Human E.coli 
OtherComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Data from ProteomeXchange, PXD ID: PXD001060. File: 00730_A11_P005652_S00_T01_R1.mzml. Published as part of Mol Cell Proteomics. 2015 Jan;14(1):205-15 . From the Abstract: {{i}} ... Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) m...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023168 | GPMDB
Resource Reanalysis 
GenomicsGlobal transcriptional profile of hESC-derived macrophage (iMAC), human monocyte-derived macrophage and THP-1 macrophage
Global transcriptional profile of hESC-derived macrophage (iMAC), human monocyte-derived macrophage and THP-1 macrophage
PRJNA575728 | ENA
OtherReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.
Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18 . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application o...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320003560 | GPMDB
Resource Reanalysis 
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
ProteomicsTIHAMAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Analysis
Protein post-translational modifications (PTMs) are pivotal molecular events in plant cells, encompassing processes such as protein folding and enzyme activation. Among these PTMs, N-glycosylation and phosphorylation hold particular importance, as they play essential roles in regulating plant growth...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-03 | PXD047065 | Pride
N-glycoproteomics Hilic Arabidopsis thaliana Imac Aba signaling Phosphoproteomics 
ProteomicsPhoX - An IMAC-enrichable Crosslinking Reagent
Chemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry. However, sub-s...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-09-13 | PXD011931 | Pride
Phosphonic acid Xlinkx Tri-functional crosslinker Structural biology Crosslinking mass spectrometry Imac enrichable crosslinker 
UnknownClinical and demographic predictors of outcomes in recent onset dilated cardiomyopathy: results of the IMAC (Intervention in Myocarditis and Acute Cardiomyopathy)-2 study.
Not available
S-EPMC6467576 | biostudies-literature
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