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ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsBioinertization of nanoLC/MS/MS systems by depleting metal ions from the mobile phases for phosphoproteomics
We have successfully bioinertized a nanoLC/MS/MS system for the highly sensitive analysis of phosphopeptides by depleting metal ions from the mobile phase. We found that not only the direct contact of phosphopeptides with metal materials, but also the indirect contact with HPLC pump through the mobi...
ORGANISM(S): Homo Sapiens (human) 
2023-03-24 | PXD034942 | JPOST Repository
OtherComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Data from ProteomeXchange, PXD ID: PXD001060. File: 00730_A11_P005652_S00_T01_R1.mzml. Published as part of Mol Cell Proteomics. 2015 Jan;14(1):205-15 . From the Abstract: {{i}} ... Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) m...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023168 | GPMDB
Resource Reanalysis 
ProteomicsSequential Phosphoproteomic Enrichment by Complementary Metal-directed Immobilized Metal Ion Affinity Chromatography: Case Study on Kinase Substrate Mapping in Human Lung Cancer Tissue
Abstract: Despite recent advances in instrumentation and analytical strategies for identification and quantitation of protein phosphorylation, methodologies to enrich the heterogeneous types of phosphopeptides are critical towards comprehensive mapping of the under-explored phosphoproteome. Taking a...
ORGANISM(S): Homo sapiens (Human) 
2014-01-29 | PXD000314 | Pride
Human lung cancer tissue Sequential imac Phosphoproteomics 
UnknownImmobilized Metal Affinity Chromatography Coupled to Multiple Reaction Monitoring Enables Reproducible Quantification of Phospho-signaling.
Not available
S-EPMC4739685 | biostudies-literature
UnknownBenchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory.
Not available
S-EPMC6231711 | biostudies-literature
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050933 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
UnknownEvaluation of quantitative performance of sequential immobilized metal affinity chromatographic enrichment for phosphopeptides.
Not available
S-EPMC3909987 | biostudies-literature
UnknownComparing multistep immobilized metal affinity chromatography and multistep TiO2 methods for phosphopeptide enrichment.
Not available
S-EPMC4766865 | biostudies-literature
UnknownImmobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success.
Not available
S-EPMC3169392 | biostudies-literature
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