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TranscriptomicsSerial analysis of chromatin occupancy (SACO) of forskolin-induced mRNA vs. an inactive control in the rat PC12 cell pheochromocytoma cell line to identify CREB targets
PC12 cells (passage 22) were cultured at 5% CO2 in a 37 C incubator. The growth medium was DMEM (high glucose, Invitrogen) supplemented with 25mM HEPES, 10% FCS, 5% FBS and 1x Pen/Strep (Invitrogen). 1x10^6 cells were starved overnight in DMEM +25 mM HEPES and treated with 10 uM 1,9 dideoxy forskoli...
ORGANISM(S): Rattus norvegicus 
2007-09-18 | E-GEOD-2071 | biostudies-arrayexpress
TranscriptomicsProfiling status epilepticus-induced changes in hippocampal RNA expression using high-throughput RNA sequencing
Status epilepticus (SE) is a life-threatening condition that can give rise to a number of neurological disorders, including learning deficits, depression, and epilepsy. Many of the effects of SE appear to be mediated by alterations in gene expression. To gain deeper insight into how SE affects the t...
ORGANISM(S): Mus musculus 
2015-08-27 | E-GEOD-72402 | biostudies-arrayexpress
TranscriptomicsTargeted deletion of miR-132/212 impairs memory and alters the hippocampal transcriptome
miR-132 and miR-212 are structurally-related microRNAs that have been found to exert powerful modulatory effects within the central nervous system (CNS). Notably, these microRNAs are tandomly processed from the same non-coding transcript, and share a common seed sequence: thus it has been difficult ...
ORGANISM(S): Mus musculus 
2016-03-01 | E-GEOD-73413 | biostudies-arrayexpress
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