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TranscriptomicsMurine CD8+ T cells: in vitro-derived vs. thymocytes
Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression prof...
ORGANISM(S): Mus musculus 
2012-09-05 | E-GEOD-37669 | biostudies-arrayexpress
TranscriptomicsCas9 target DNA specificity in vitro and in vivo (S. cerevisiae)
Cas9, a CRISPR RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although Cas9 offers remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of Cas9 target::homology requirements, w...
ORGANISM(S): synthetic construct 
2016-06-10 | E-GEOD-79667 | biostudies-arrayexpress
ProteomicsAn in vitro model maintaining taxon-specific functional activities of the gut microbiome
We developed and validated a 96-deep well plate-based culturing model named Mipro to maintain individuals’ microbiomes. The Mipro model quintupled viable bacteria count while maintained the functional and compositional profiles of individuals’ gut microbiomes.
ORGANISM(S): Escherichia coli 
2019-08-16 | PXD013600 | Pride
Gut microbiome In vitro culturing Metaproteomics 
GenomicsIn vitro generation of brain regulatory T cells by co-culturing with astrocytes
In vitro generation of brain regulatory T cells by co-culturing with astrocytes
PRJNA838355 | ENA
ProteomicsIn vitro evaluation of MiPro cultuturing model
The human stool samples were collected for establishing and evaluating the MiPro culturing model. Metaproteomics were used to evaluate the model performance in maintaining microbiome composition and functions.
ORGANISM(S): Escherichia coli 
2019-08-16 | PXD010134 | Pride
In vitro Culture Microbiome Human 
TranscriptomicsExpression data from in vitro versus in vivo differentiated Th17 cells
In vitro differentiated Th17 have a distinct expression profile compared to in vivo differentiated Th17 Several conditions of Th17 cells were tested, with 2-3 replicates per condition.
ORGANISM(S): Mus musculus 
2015-04-18 | E-GEOD-68017 | biostudies-arrayexpress
MetabolomicsElucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells

Environmental exposure to xenoestrogens, i.e., chemicals that imitate the hormone 17β-estradiol, has the potential to influence hormone homeostasis and action. Detailed knowledge of xenobiotic biotransformation processes in cell models is key when transferring knowledge learned from in vitro<...

2023-10-26 | MTBLS2716 | MetaboLights
ProteomicsProtein profile of mouse ovarian follicles grown in vitro
This project presents a protein catalog of the in vitro growing mouse ovarian follicle and it provides insights about proteomics throughout in vitro follicle development.
ORGANISM(S): Mus musculus (Mouse) 
2017-10-31 | PXD006227 | Pride
Follicles Lc-msms Mouse 
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells [CGH]
Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from re...
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31256 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse CD8+ T cells from wild type and Elf4-/- mice left untreated or activated in vitro by culturing on anti-CD3 coated plates and anti-CD28 in media
Transcription factors that regulate quiescence, proliferation, and homing of lymphocytes are critical for effective immune system function. In the present study, we demonstrated that the transcription factor ELF4 directly activates the tumor suppressor KLF4 downstream of T cell receptor (TCR) signal...
ORGANISM(S): Mus musculus 
2009-03-28 | E-GEOD-15324 | biostudies-arrayexpress
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