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TranscriptomicsExpression Profiles of HepG2 cells treated with genotoxic and non-genotoxic agents
The lack of accurate in vitro assays for predicting in vivo toxicity of chemicals together with new legislations demanding replacement and reduction of animal testing has triggered the development of alternative methods. This study aimed at developing a transcriptomics-based in vitro prediction assa...
ORGANISM(S): Homo sapiens 
2012-08-02 | E-GEOD-28878 | biostudies-arrayexpress
TranscriptomicsCas9 target DNA specificity in vitro and in vivo (S. cerevisiae)
Cas9, a CRISPR RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although Cas9 offers remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of Cas9 target::homology requirements, w...
ORGANISM(S): synthetic construct 
2016-06-10 | E-GEOD-79667 | biostudies-arrayexpress
TranscriptomicsEffects of in vitro maturation on oocyte gene expression
In vitro oocyte maturation (IVM) holds great promise as a tool for enhancing clinical treatment of infertility, enhancing availability of non human primates for development of disease models, and facilitating endangered species preservation. However, IVM outcomes have remained significantly below s...
ORGANISM(S): Macaca mulatta 
2009-05-20 | E-GEOD-11895 | biostudies-arrayexpress
TranscriptomicsMethylation profiling by array of blood cells from patients with tartrate resistant acid phosphatase deficiency
The autosomal recessive immuno-osseus dysplasia spondyloenchondrodysplasia (SPENCD) is characterised by the variable combination of metaphyseal and vertebral bone lesions, immune dysfunction with features of both autoimmunity and immunodeficiency, and neurological involvement including developmental...
ORGANISM(S): Homo sapiens 
2011-05-05 | E-MEXP-2699 | biostudies-arrayexpress
ProteomicsHigh-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates
Abnormalities in the FLT3 signaling pathway play an integral role in AML disease relapse and drug resistance. Developing new and specific FLT3 tyrosine kinase inhibitors for use in combination to induction therapy is an important step to reduce disease relapse and achieve clinical remission. To deve...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD011478 | Pride
Kinase assay linked with phosphoproteomics Flt3 Acute myeloid leukemia Mass spectrometry Phosphoproteomics 
TranscriptomicsMurine CD8+ T cells: in vitro-derived vs. thymocytes
Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression prof...
ORGANISM(S): Mus musculus 
2012-09-05 | E-GEOD-37669 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse dendritic cell differentiation from dendritic cell progenitor in vitro
FLT3+CD11b+ DC progenitor was amplified in vitro from mouse bone marrow. DC were differentiated using GM-CSF and analyzed at day 7 and day 10 of differentiation and after 16h TNFalpha stimulation at day 10
ORGANISM(S): Mus musculus 
2008-06-14 | E-GEOD-575 | biostudies-arrayexpress
TranscriptomicsIn-Vitro Cell Adhesion Assay
The adhesion of monocytic cells to the M-^SdysfunctionalM-^T endothelium constitutes a critical step in the initiation of atherosclerotic plaque formation. Cigarette smoke (CS) has been shown to contribute to the monocyte-endothelial adhesion process. Yet, the complex mechanisms underlying this even...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-MTAB-1173 | biostudies-arrayexpress
ProteomicsLens Disulfide crosslink
Low glutathione levels are associated with crystallin oxidation in age-related nuclear cataract (ARNC). To understand the role of cysteine residue oxidation, we used the novel approach of comparing human cataracts with glutathione-depleted LEGSKO mouse lenses for intra- vs. intermolecular disulfide ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2016-07-20 | MSV000079952 | MassIVE
GenomicsTo determine the mutational impact of the in vitro culture, clonal human adult and pluripotent stem cell lines were subjected to a second clonal step after 3 months of culture. These subclones were whole genome sequenced to identify all the mutations that accumulated during the 3 month culture period.
Genetic changes acquired during culture pose a potential risk for the successful application of stem cells. To assess the risk of in vitro expansion on mutation accumulation we have performed whole genome sequencing of clonally expanded human induced pluripotent stem cells (iPS cells) and adult stem...
EGAS00001002955 | EGA
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