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ProteomicsIn-Depth Comparison of Reagent-Based Digestion Methods and Two Commercially Available Kits for Bottom-Up Proteomics
We assessed two physical disruption methods to homogenize cells —sonication and BeatBox—alongside four digestion protocols. Two of them are lab-reagent strategies: urea-based, and SDC (Sodium deoxycholate)-based in-solution digestion, and two are commercially available kits: the EasyPep kit from The...
ORGANISM(S): Homo sapiens (Human) 
2026-01-07 | PXD056409 | Pride
In-solution digestion Lc-ms Proteomics Proteolysis Bottom-up proteomics 
ProteomicsComparison of in-solution and SP3 sample processing workflows on HeLa cell samples
Comparison of in-solution and single-pot, solid-phase-enchanced sample preparation (SP3) sample processing workflows on HeLa cell samples, using different lysis buffers based on SDS and guanidine hydrochloride, for optimal analysis by liquid chromatography mass spectrometry (LC-MS)
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031918 | Pride
Proteomics Lc-ms Hela Sp3 In-solution digestion 
ProteomicsSerial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis
Advancement in mass spectrometry has revolutionised the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus, an...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Mus musculus (Mouse) 
2020-04-30 | PXD017513 | Pride
Glycosaminoglycans Proteomics Mass spectrometry Glycomics Proteoglycans Fasp Extracellular matrix. 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030797 | Pride
Speed Plasma Kidney Sheep Tripletof S-trap Dda 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030796 | Pride
Speed Plasma Kidney Sheep Swath-ms Tripletof S-trap 
ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
UnknownInvestigation of Tryptic Protein Digestion in Microdroplets and in Bulk Solution.
Not available
S-EPMC10512443 | biostudies-literature
ProteomicsQuantitative assessment of the effects of trypsin digestion methods on immunoprecipitation-mass spectrometry-based protein-protein interaction analysis
Immunoprecipitation-mass spectrometry (IP-MS) has become the method of choice for discovering protein-protein interaction (PPI) under native conditions. The success of the IP-MS depends on the efficiency of trypsin digestion and recovery of the tryptic peptides for MS analysis. Several different pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2017-07-25 | PXD005984 | Pride
Rela interactome Trypsin digestion Protein-protein interaction 
UnknownQuantitative assessment of in-solution digestion efficiency identifies optimal protocols for unbiased protein analysis.
Not available
S-EPMC3790306 | biostudies-literature
ProteomicsQuantitative assessment of the effects of trypsin digestion methods on immunoprecipitation-mass spectrometry-based protein-protein interaction analysis - part2
Immunoprecipitation-mass spectrometry (IP-MS) has become the method of choice for discovering protein-protein interaction (PPI) under native conditions. The success of the IP-MS depends on the efficiency of trypsin digestion and recovery of the tryptic peptides for MS analysis. Several different pro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-26 | PXD006739 | Pride
Protein-protein interaction Trypsin digestion Lc-ms/ms 
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