To test TWEAK/Fn14 pathway and relative agents in chronic TNBS colitis Chronic colitis was induced by rectal injection of TNBS with ethanol for 6 weeks in WT or FN14 KO BALB/c mice. Colons were harvested for gene profiling.
To identify the proteins interacting with Gore-tex/Osiris23, affinity purification mass spectrometry (AP-MS) was performed using S2 cells transfected with HA or Flag-tagged Gore-tex/Osiris23 expression vectors. PTM analysis was also performed to determine the ubiquitination sites of Gore-tex/Osiris2...
