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ProteomicsDirect 3D sampling of the embryonic mouse head: Layer-wise nanosecond infrared laser (NIRL) ablation from scalp to cortex for spatially resolved proteomics
Common workflows in bottom-up proteomics require homogenization of tissue samples giving to get access to the biomole-cules within the cells. The homogenized tissue samples often contain many different cell types, thereby representing an average of the natural proteome composition and rare cell type...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD043011 | Pride
Nirl ablation Spatial proteomics Tissue sampling Bottom-up proteomics 
ProteomicsSampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenizatio...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-17 | PXD027700 | Pride
Laser ablation Tissue sampling  nanosecond infrared laser Proteomics  mass spectrometry 
ProteomicsWavelength Dependence of Intact Protein Extraction Using Femtosecond Laser Ablation
Raw files for the paper named: Wavelength Dependence of Intact Protein Extraction Using Femtosecond Laser Ablation
ORGANISM(S): Mastigocladopsis Repens Rhodopsin Equine Myoglobin Lysozyme From Chicken Egg White 
2025-03-04 | MSV000097234 | MassIVE
ProteomicsTissue sampling and homogenization with NIRL enables spatially resolved cell layer specific proteomic analysis of the murine intestine
For investigating the molecular physiology and pathophysiology in organs the most exact data will be obtained, if not organ specific cell lines are analysed or the whole organ is homogenized, followed by the analysis of its biomolecules, but if the morphological organisation of the organ can be addr...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-22 | PXD033584 | Pride
Colon 3d Mouse Laser-ablstion Lc-ms/ms 
UnknownTransmissive Mode Laser Micro-Ablation Performance of Ammonium Dinitramide-Based Liquid Propellant for Laser Micro-Thruster.
Not available
S-EPMC10303727 | biostudies-literature
UnknownA Comparative Study of Laser-Induced Graphene by CO2 Infrared Laser and 355 nm Ultraviolet (UV) Laser.
Not available
S-EPMC7764730 | biostudies-literature
ProteomicsDifferential Proteome Analysis of Human Neuroblastoma Xenograft Primary Tumors and Matched Spontaneous Distant Metastases
Metastasis formation is the major cause for cancer-related deaths and the underlying mechanisms remain poorly understood. In this study we describe spontaneous metastasis xenograft mouse models of human neuroblastoma used for unbiased identification of metastasis-related proteins by applying an infr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-09-24 | PXD009744 | Pride
Bioluminescence imaging Proteome analysis Spontaneous metastasis Infrared laser ablation 
UnknownMid-infrared absorption by soft tissue sarcoma and cell ablation utilizing a mid-infrared interband cascade laser.
Not available
S-EPMC8058894 | biostudies-literature
UnknownSingle-Shot Multi-Stage Damage and Ablation of Silicon by Femtosecond Mid-infrared Laser Pulses.
Not available
S-EPMC6934619 | biostudies-literature
ProteomicsHomogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization
Tissue samples from human tonsils and rat pancreas were subjected to a novel laser-based homogenization method, the PIRL-DIVE homogenization, and to a conventional mechanical tissue homogenization procedure (bead mill and a cryo-grinder). The protein species composition of the protein extracts were ...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2016-01-25 | PXD003434 | Pride
Protein species Sample preparation Proteolysis Pirl Tissue homogenization Lc-msms Pirl-dive 
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