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MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
ProteomicsGlobal quantification of intact proteins via chemical isotope labeling and mass spectrometry
Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation effici...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-30 | PXD012526 | Pride
Intact protein quantification Ptop Chemical isotope labeling Highest isotopic peak Mass spectrometry 
ProteomicsOptimization of Higher-Energy Collisional Dissociation Fragmentation Energies for Intact Protein-level Tandem Mass Tag Labeling
Isobaric chemical tag labeling (e.g., iTRAQ and TMT) is a commonly used approach in quantitative proteomics research. Typically, peptides are covalently labeled with isobaric chemical tags, and quantification is enabled through detection of low-mass reporter ions generated after MS2 fragmentation. R...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD037491 | Pride
Protein-level tmt labeling  top-down proteomics  quantitative proteomics  hcd 
UnknownImpact of Phosphorylation on the Mass Spectrometry Quantification of Intact Phosphoproteins.
Not available
S-EPMC6138620 | biostudies-literature
ProteomicsHigh-Throughput Intact Glycopeptide Quantification Strategy with Targeted-MS (HTiGQs-Target) Reveals Site-Specific IgG N-Glycopeptides as Biomarkers for Hepatic Disorder Diagnosis and Staging
We developed a high-throughput targeted quantification strategy for intact glycopeptides (HTiGQs-Target) to diagnose and differentiate the malignancy of liver diseases. The method involves two-step labeling. This step derivatization makes the same parent ions in different samples produce sufficient ...
ORGANISM(S): Homo Sapiens 
PXD042528 |
UnknownQuantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins.
Not available
S-EPMC3440571 | biostudies-literature
UnknownImmunoaffinity Intact Top-Down Mass Spectrometry for Quantification of Neuron-Specific Enolase Gamma, a Low-Abundance Protein Biomarker.
Not available
S-EPMC11740178 | biostudies-literature
ProteomicsIntact Protein extraction using Liquid extraction surface analysis (LESA)
Development of a new technique of extraction of intact protein on localized region of tissue section using liquide extraction surface analysis.
ORGANISM(S): Rattus norvegicus (Rat) 
2016-03-14 | PXD001925 | Pride
Lesa Intact proteins extraction 
UnknownCharacterization and quantification of intact 26S proteasome proteins by real-time measurement of intrinsic fluorescence prior to top-down mass spectrometry.
Not available
S-EPMC3594244 | biostudies-literature
ProteomicsAn integrated MS data processing strategy for fast identification, in-depth and reproducible quantification of protein O-glycosylation in large cohorts of human urine samples
Protein O-glycosylation has long been recognized to be closely associated with many diseases, particularly with tumor proliferation, invasion and metastasis. The ability to efficiently profile the variation of O-glycosylation in large-scale clinical samples provides an important approach for the devel...
ORGANISM(S): Homo sapiens (Human) 
2020-02-04 | PXD015987 | Pride
O-glycopeptide  urine  sialic acid 
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