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Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-based phosphoproteomics for signaling pathway reconstitution is challenging when analyzing tiny sample amounts. We present a novel hybrid data-independent acquisition (DIA) strategy (hybrid-DIA) that combines...
ORGANISM(S): Homo sapiens (Human) 
2023-05-18 | PXD038947 | Pride
Quality control (QC) in mass spectrometry (MS)-based proteomics is mainly based on data-dependent acquisition (DDA) analysis of standard samples. Here, we collected 2638 files acquired by data independent acquisition (DIA) and paired DDA files from mouse liver digests using 21 mass spectrometers acr...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-08 | PXD052871 | Pride
Analysis of large-scale data-independent acquisition mass spectrometry (DIA-MS) metaproteomics data remains a computational challenge. Here, we present a computational pipeline called metaExpertPro for metaproteomics data analysis. This pipeline encompasses spectral library generation using data-dep...
ORGANISM(S): Homo sapiens (Human) 
2024-09-28 | PXD051104 | Pride
Targeted proteomics has been playing an increasingly important role in hypothesis-driven protein research and clinical bi-omarker discovery. We have previously created a workflow, Tomahto, to enable real-time targeted pathway proteomics assays using two-dimensional multiplexing technology. Coupled w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-08-14 | MSV000095604 | MassIVE
We introduce FluoroMatch, which automates file conversion, chromatographic peak picking, blank feature filtering, PFAS annotation based on precursor and fragment masses, and annotation ranking. The software library currently contains about 7000 PFAS fragmentation patterns based on rules derived from...
ORGANISM(S): Na 
2023-03-30 | MSV000091594 | MassIVE
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