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MetabolomicsInositol triphosphate-triggered calcium release blocks lipid exchange at endoplasmic reticulum-Golgi contact sites
Vesicular traffic and membrane contact sites between organelles enable the exchange of proteins, lipids, and metabolites. Recruitment of membrane tethers to contact sites between the endoplasmic reticulum (ER) and the plasma membrane is often triggered by calcium. In contrast, we reveal here a funct...
2021-05-06 | MTBLS2444 | MetaboLights
MetabolomicsQuantification of Dissolved Metabolites in Environmental Samples through Cation-Exchange Solid Phase Extraction (CX-SPE) paired with Liquid Chromatography-Mass Spectrometry
Not available
ST002292 | MetabolomicsWorkbench
MetabolomicsApoplastic metabolomics reveals sugars as mesophyll messengers regulating guard cell ion transport under red light
Here we sought to identify mesophyll messenger(s) that promote stomatal opening under red light. We first established a method to isolate leaf apoplastic fluids from Arabidopsis thaliana and Vicia faba and ascertained that the apoplastic fluid enhances red light-induced stomatal opening. We then app...
2025-06-23 | MTBLS5383 | MetaboLights
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
GenomicsLectin microarray analysis of murine CTL EVs separated by ion exchange method
Lectin microarray analysis of murine CTL EVs separated by ion exchange method
PRJNA612028 | ENA
GenomicsmiRNA microarray analysis of murine CTL EVs separated by ion exchange method [miRNA]
miRNA microarray analysis of murine CTL EVs separated by ion exchange method [miRNA]
PRJNA612029 | ENA
GenomicsmRNA microarray analysis of murine CTL EVs separated by ion exchange method [mRNA]
mRNA microarray analysis of murine CTL EVs separated by ion exchange method [mRNA]
PRJNA612023 | ENA
ProteomicsQuantative pTyr protein complex
Our current research focuses on the complex-centric quantification of pTyr protein complexes. We initially constructed a tri-functional probe based on pTyr superbinder (SH2-S) for the enrichment of pTyr protein complexes. The pTyr protein complexes were separated by ion exchange chromatography (IEC)...
ORGANISM(S): Homo Sapiens 
2024-05-31 | PXD052742 |
MetabolomicsLiquid chromatography coupled to high-resolution mass spectrometry metabolomics: A useful tool for investigating tumor secretome based on a three-dimensional co-culture model

Three-dimensional (3D) cell culture technologies, which more closely mimic the complex microenvironment of tissue, are being increasingly evaluated as a tool for the preclinical screening of clinically promising new molecules, and studying of tissue metabolism. Studies of metabolites released int...

2023-12-12 | MTBLS5375 | MetaboLights
ProteomicsOnline Mixed-Bed Ion Exchange Chromatography for Native Top-Down Proteomics of Complex Mixtures
Native top-down mass spectrometry (nTDMS) allows characterization of protein structure and noncovalent interactions with simultaneous sequence mapping and proteoform characterization. The majority of nTDMS studies utilize purified recombinant proteins, with significant challenges hindering applicati...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-05-17 | MSV000094787 | MassIVE
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