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ProteomicsON THE ACCURACY, PRECISION AND STATISTICAL SIGNIFICANCE OF COMPRESSED RATIOS IN ISOBARIC LABELLING
Despite the outstanding advantages that isobaric labelling has to offer in quantitative proteomics, its precision and accuracy are undermined by ratio compression, which is consequence of the co-isolation of peptides within a selected isolation window. Here, using the two-proteomes approach we inves...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2016-08-08 | PXD003640 | Pride
Isobaric labelling Statistics Accuracy Ratio compression Precision 
ProteomicsA Collision-induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-based Quantification in Proteomics
Fragment ion-based proteome quantification methods quantify peptides based on unique peptide fragment ions avoiding the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a novel approach that relies on a collision-induced dissoci...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-08-17 | PXD018790 | Pride
Isotopic labelling Proteomics Cid cleavable tag Fragment ion-based quantification Isobaric labelling 
ProteomicsEvaluation of a Dual Isolation Width Acquisition (DIWA) method for isobaric labelling ratio decompression
Stable isotope labelling of peptides using isobaric reagents such as iTRAQ and TMT enables the multiplexed analysis of proteomes with deep quantitative coverage. Isobaric tagging demonstrates high precision but imperfect accuracy due to ratio underestimation caused by co-fragmentation of ions with m...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2019-01-07 | PXD010571 | Pride
Method development Tmt Msms Diwa 
ProteomicsSMD-IPTL 4-plex yeast and BSA-yeast
Isobaric peptide termini labeling (IPTL) is an attractive protein quantification method, because it provides more accurate and reliable quantification information than traditional isobaric labelling methods (TMT, iTRAQ) by making use of the entire fragment ion series instead of only a single reporte...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-04-27 | PXD018589 | Pride
Smd-iptl 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsTurboRAW2MGF: Removing isobaric-related ions improves the peptide/protein identification
Isobaric labelling technique coupled with high resolution mass spectrometry has been widely employed in the proteomics workflows requiring relative quantification. For each high resolution tandem mass spectrum (MS/MS), it can be used not only to quantify the peptide from different samples by reporte...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2015-01-30 | PXD000994 | Pride
Isobaric labelling Ltq-orbitrap Preprocessing Peptide identification Itraq 
ProteomicsDetecting single amino acids and small peptides by combining isobaric tags and peptidomics
Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment we found that by combining isobaric t...
ORGANISM(S): Homo sapiens (Human) 
2019-06-26 | PXD012872 | Pride
Cerebrospinal fluid Tmt Isobaric peptidomics Peptidomics Tandem mass tag 
ProteomicsPhoXplex: Combining phospho-enrichable crosslinking with isobaric labelling for quantitative proteome-wide mapping of protein interfaces
Integrating crosslinking mass spectrometry (XL-MS) into structural biology workflows provides valuable information about the spatial arrangement of amino acid stretches, which can guide elucidation of protein assembly architecture. Additionally, combination of XL-MS with peptide quantitation techniq...
ORGANISM(S): Homo sapiens (Human) 
2024-10-21 | PXD052480 | Pride
Isobaric labelling Phospho-enrichable crosslinking 
ProteomicsTMTpro: Design and initial evaluation of a novel Proline-based isobaric 16-plex Tandem Mass Tag reagent set
The design and synthesis of a novel proline-reporter-based isobaric Tandem Mass Tag 16 tag set (TMTpro) was carried out and the data uploaded here is a comparison of the performance of the new TMTpro tags with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. Da...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Rattus norvegicus (Rat) 
2019-11-25 | PXD014750 | Pride
Tmt Tmtpro 
UnknownIsobaric 6-plex and tosyl dual tagging for the determination of positional isomers and quantitation of monounsaturated fatty acids using rapid UHPLC-MS/MS.
Not available
S-EPMC12711191 | biostudies-literature
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