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MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation - DIA lipid mediator validation

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1382 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method development)
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS826 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Method quantification)
One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas...
2019-11-15 | MTBLS848 | MetaboLights
MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation, Targeted Phospho and Glycerolipid Profiling

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1369 | MetaboLights
MetabolomicsUntargeted In Silico Compound Classification - A Novel Metabolomics Method to Assess the Chemodiversity in Bryophytes

In plant ecology, biochemical analyses of bryophytes and vascular plants are often conducted on dried herbarium specimen as species typically grow in distant and inaccessible locations. Here, we present an automated in silico compound classification framework to annotate metabolites using an unta...

2026-01-30 | MTBLS851 | MetaboLights
TranscriptomicsTranscription profiling of human peripheral blood samples collected into PAXgene or Tempus tubes vs. the same 5 healthy control samples drawn in Li Heparin Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (direct, heparin
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of R...
ORGANISM(S): Homo sapiens 
2008-11-13 | E-GEOD-12710 | biostudies-arrayexpress
TranscriptomicsAn improved method for the isolation of endometrial epithelial and stromal cells from the total hysterectomy specimen
We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from hysterectomy specimens. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtr...
ORGANISM(S): Homo sapiens 
2016-06-14 | E-GEOD-73090 | biostudies-arrayexpress
ProteomicsA Novel Method for Isolating Whole Protein from Human Cranial Bone
The presence of the extracellular matrix within human bone limits the applicability of conventional protocols for protein extraction. As a result, complete and accurate characterization of human bone proteome is yet to be performed, and as a result, several bone-related diseases such as craniosynost...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080697 | MassIVE
TranscriptomicsTranscription profiling of leukemia samples prepared using different RNA isolation procedures
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Fo...
ORGANISM(S): Homo sapiens 
2008-11-06 | E-GEOD-7757 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human peripheral blood samples using different collection protocols - superseries of GSE12709, GSE12710
This SuperSeries is composed of the following subset Series:; GSE12709: Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (PHA); GSE12710: Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (direct, hepari...
ORGANISM(S): Homo sapiens 
2008-11-13 | E-GEOD-12711 | biostudies-arrayexpress
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