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MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation - DIA lipid mediator validation

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1382 | MetaboLights
ProteomicsIsolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803
The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This p...
ORGANISM(S): Synechocystis Sp. Pcc 6803 (ncbitaxon:1148) 
2022-06-03 | MSV000089593 | MassIVE
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells
This SuperSeries is composed of the following subset Series: GSE31255: Isolation and in vitro expansion of human colonic stem cells [Expression profile] GSE31256: Isolation and in vitro expansion of human colonic stem cells [CGH] Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31257 | biostudies-arrayexpress
TranscriptomicsIsolation and in vitro expansion of human colonic stem cells [CGH]
Tissue for normal colonic stem cell isolation was obtained via colectomy from a colorectal cancer patient. It was shown that the resected tissue and the in vitro-cultured organoids grown from tissue-derived adult stem cells do not harbor chromosomal alterations. For that purpose, genomic DNA from re...
ORGANISM(S): Homo sapiens 
2011-11-22 | E-GEOD-31256 | biostudies-arrayexpress
TranscriptomicsAn improved method for the isolation of endometrial epithelial and stromal cells from the total hysterectomy specimen
We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from hysterectomy specimens. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtr...
ORGANISM(S): Homo sapiens 
2016-06-14 | E-GEOD-73090 | biostudies-arrayexpress
ProteomicsA COFRADIC protocol to study protein ubiquitination
We here apply the COmbined FRActional DIagonal Chromatography (COFRADIC) technology to enrich for ubiquitinated peptides and identify sites of ubiquitination by mass spectrometry. Our technology bypasses the need to ectopically overexpress tagged variants of ubiquitin and the use of sequence-biased ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080780 | MassIVE
GenomicsOptimized Protocol for Culturing Porcine Retinal Explants and RNA Isolation
Optimized Protocol for Culturing Porcine Retinal Explants and RNA Isolation
PRJNA1416961 | ENA
GenomicsIsolation protocol of viable human central nervous system T cells
Isolation protocol of viable human central nervous system T cells
PRJNA1322312 | ENA
ProteomicsIsolation method and characterization of outer membranes vesicles of Helicobacter pylori grown in a chemically defined medium
Outer membrane vesicles (OMVs) are small vesicles constitutively shed by all Gram-negative bacterium, which have been proposed to play a role in Helicobacter pylori persistence and pathogenesis. The methods currently available for the isolation of H. pylori OMVs are diverse and time-consuming, raisi...
ORGANISM(S): Helicobacter pylori 26695 
2021-05-31 | PXD025393 | Pride
Proteomics Helicobacter pylori Bacterial outer membrane vesicles Bacteria viability Defined medium Isolation protocol 
TranscriptomicsTranscription profiling of human peripheral blood samples collected into PAXgene or Tempus tubes vs. the same 5 healthy control samples drawn in Li Heparin Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (direct, heparin
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of R...
ORGANISM(S): Homo sapiens 
2008-11-13 | E-GEOD-12710 | biostudies-arrayexpress
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