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ProteomicsDifferential solubilization and top-down proteomics with FAIMS control experiments applied to mouse brain
5-35 mg brain tissue plugs from mouse left or right hemisphere were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homogenization buffer (H...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-12-25 | MSV000093727 | MassIVE
ProteomicsLarge scale, quantitative top-down proteomic analysis of human frontal gyrus brain tissue samples in the context of Alzheimer's disease
15-35 mg brain tissue plugs derived from dorsal lateral prefrontal cortex (DLPFC) were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homog...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-26 | MSV000093728 | MassIVE
ProteomicsCharacterization of cytokine treatment on human pancreatic islets by top-down proteomics
Top-down proteomic data from 6 human islet donors; islets were treated with interferon gamma (IFN-gamma) and interleukin-1 beta (IL-1 beta). Time points include t=0 (control) and t=24 hours for each donor. Samples were reduced and alkylated, then analyzed by LC-MS/MS. Data was searched with TopPIC u...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-05-06 | MSV000097810 | MassIVE
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