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Treatment of the CLB-GA cell line with complementary ALK, MEK, PI3K/mTOR or RET inhibitors (and DMSO (VWR) as control) was performed in duplicate for each drug using the GI50 concentrations (see further): 0.5M-k_M Crizotinib (Pfizer/Sigma-Aldrich); 0.06M-k_M X-396 (VWR); 0.2M-k_M LDK378 (Hoelzel Bio...
ORGANISM(S): homo sapiens 
Establishment of inducible SK-N-AS ALKwt, ALKF1174L and ALKR1275Q cell lines Human neuroblastoma SK-N-AS cells were electroporated with pcDNA6/TR (Invitrogen) using the NeonM-eM-( Transfection System (Life Techologies). Single cell clones were generated using blasticidin (7.5M-eM-5g/ml) and limited ...
ORGANISM(S): homo sapiens 

Longitudinal metabolomics and lipidomics analysis was carried out in the blood plasma of mice injected intramuscularly with venoms of the viperid species Bothrops asper and Daboia russelii. Blood samples were collected 1, 3, 6 and 24 h after venom injection, and a control group ...

2022-09-21 | MTBLS5430 | MetaboLights
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