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ProteomicsQuantitative proteomics reveals extensive lysine ubiquitination in Arabidopsis thaliana
In this study, we describe an antibody-based approach to enrich ubiquitinated peptides from vegetative tissues for detection via peptide mass spectrometry. This enrichment method can be coupled with isobaric labeling to enable quantification from up to 18-multiplexed samples. This approach identifie...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-07-31 | MSV000095496 | MassIVE
ProteomicsAlkylation reageant comparaison
Comparison of off target alkylation using chloroacetamide (CAA) and iodoacetamide (IAA). The peptide preparation was performed side by side, following identical methods except the alkylation treatment with either IAA or CAA. None of the comparison of off-targets on between IAA and CAA showed a signi...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-07-31 | MSV000095495 | MassIVE
ProteomicsUbiquitylome analysis of Arabidopsis roots.
Ubiquitylome analysis of Arabidopsis roots. 10 day old Arabidopsis roots were treated with mock, DMSO, or bortezomib for 3 hours. Root tissue was processed using the phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Ubiquitin remnant peptides were enriched using an anti-diglyci...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000086156 | MassIVE
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