The antibiotics resistance of Edwardsiella tarda is prevailing, seeking a novel antimicrobial strategies extremely urgent. Lysine acylation has been proved to play important role on the bacterial physiological function while its role on bacterial antibiotics resistance mechanism still largely unclea...
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
