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TranscriptomicsTwo transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon [ChIP-chip data]
Because iron toxicity and deficiency are equally life threatening, maintaining intracellular iron levels within a narrow optimal range is critical for nearly all known organisms. However, regulatory mechanisms that establish homeostasis are not well understood in organisms that dwell in environments...
ORGANISM(S): Halobacterium sp. NRC-1 
2011-06-02 | E-GEOD-29705 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and bop perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of bop was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7717 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 General transcription factor perturbation
Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7713 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and ura3 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illu...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7715 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen and light perturbation
A 50ml culture of Halobacterium NRC-1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7729 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and phr1/phr2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletions of phr1 and phr2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a sta...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7716 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and phr1 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of phr1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7714 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and boa1 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of boa1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7723 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and phoR perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of phoR was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7730 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and boa4 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of boa4 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7725 | biostudies-arrayexpress
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