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TranscriptomicsTopaz1, a germ cell specific factor essential for male meiotic progression.
Testicular gene expression changes with loss of Topaz1 Total RNA was extracted from 15 or 20 dpp of WT or Topaz1-/- (KO) mouse testis (C57Bl6), There were 3 biological repeats by conditions.
ORGANISM(S): Mus musculus 
2015-11-10 | E-GEOD-67229 | biostudies-arrayexpress
GenomicsThe DNA methylome of Epiblast Stem Cells and Embryonic Stem Cells is distinct and not fully reversible
Embryonic Stem Cells (ESCs) and Epiblast Stem Cells (EpiSCs) are the in vitro representants of naM-CM-/ve and primed pluripotency, respectively. It is currently unclear how their epigenome underpin the phenotypic and molecular characteristics of these states of pluripotency. Here, we performed the f...
ORGANISM(S): Mus musculus 
2014-04-28 | E-GEOD-47793 | biostudies-arrayexpress
TranscriptomicsTranscriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum
Comparison of the transcriptional response to the bacteria in the pronephros of a susceptible and a resistant line by micro-array analysis five days after infection Four to seven individual fishes have been analyzed for each experimental condition : Infected or Control ; Susceptible or resistant lin...
ORGANISM(S): Oncorhynchus mykiss 
2013-01-02 | E-GEOD-35448 | biostudies-arrayexpress
TranscriptomicsAnalyses of lung early responses to RSV in the neonatal environment reveal key roles for DC and type I IFN in controlling immunopathologic imprinting.
The gene expression profile of neonatal versus adult lung, 2 days after an RSV or mock infection was analyzed using whole mouse genome agilent microarrays. Total lung RNA were extracted, labeled with Cy3 and hybridized on Agilent G4122F slides. We used 4 biological replicates per condition.
ORGANISM(S): Mus musculus 
2015-12-21 | E-GEOD-42759 | biostudies-arrayexpress
TranscriptomicsGene expression of murine B cells activated via TLR4 or TLR4 + CD40: Group1 naM-CM-/ve B cells, Group2 LPS activated B cells 24h, Group3 LPS + anti-CD40 activated B cells 24h, Group4 LPS activated B cells 72h, and Group5 LPS + anti-CD40 activated B cells 72h.
Murine B cells can be activated via the surface receptors TLR4 and CD40. For a global assessment of differences in gene expression between these two different modes of B cell activation a genome wide transcriptome analysis was performed. In order to dissect different gene expression profiles of B ce...
ORGANISM(S): Mus musculus 
2014-02-24 | E-GEOD-35998 | biostudies-arrayexpress
TranscriptomicsDendritic cell subtypes from lymph nodes and blood show contrasted gene expression programs upon Bluetongue Virus infection
The gene expression in conventional and plasmacytoid dendritic cells (cDC and pDC respectively) during Bluetongue virus (BTV) infection in sheep depends on the lymphoid compartment and suggest that these cell types have a role in the physiopathology We analyzed the gene expression in lymph node pDC...
ORGANISM(S): Ovis aries 
2013-07-09 | E-GEOD-48450 | biostudies-arrayexpress
TranscriptomicsMolecular and functional features demarcate embryonic and induced pluripotent stem cells in rabbits
Little is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived and characterized two types of rabbit PSCs from the same breed of New Zealand White rabbits: four lines of embryonic stem cells (rbESCs), and three lines of induced PS...
ORGANISM(S): Oryctolagus cuniculus 
2013-01-11 | E-GEOD-43403 | biostudies-arrayexpress
TranscriptomicsFemale- and male-specific transcriptomic and epigenetic placental signatures in response to a maternal high fat diet in mice
Overnutrition during pregnancy inM-oM-,M-^Buences the future health of the offspring, with outcomes differing depending on the childM-bM-^@M-^Ys sex. The placenta is involved in the programming of obesity, type 2 diabetes and cardiovascular disease. Sex-specific adaptation of the placenta may be cen...
ORGANISM(S): Mus musculus 
2012-03-31 | E-GEOD-29585 | biostudies-arrayexpress
TranscriptomicsPig skin includes dendritic cells subsets homologous to human CD1a and CD14 dendritic cells presenting different migrating behaviors and T CELL activation capacities.
Characterization of swine skin DC subpopulations by means of global expression analysis. Three sets of swine skin DC plus peripheral blood B cells and monocytes (1 color hybridizations)
ORGANISM(S): Sus scrofa 
2014-12-19 | E-GEOD-53500 | biostudies-arrayexpress
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