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TranscriptomicsCellular aging can be monitored by DNA-methylation changes at specific CpG sites
Long-term culture associated changes need to be considered for quality control of cell preparations – especially in cellular therapy. Here we describe a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used ...
ORGANISM(S): Homo sapiens 
2012-05-21 | E-GEOD-29661 | biostudies-arrayexpress
TranscriptomicsMatrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [DNAm profiling]
Matrix elasticity influences differentiation of mesenchymal stem cells (MSCs) but it is unclear if these effects are only transient - while the cells reside on the substrate - or if they reflect persistent lineage commitment. In this study, MSCs were continuously culture-expanded in parallel either ...
ORGANISM(S): Homo sapiens 
2014-07-29 | E-GEOD-55888 | biostudies-arrayexpress
TranscriptomicsMethylation Pattern of Human Dermal Fibroblasts
In this study, we have analyzed DNA methylation changes upon aging of human dermal fibroblasts by using the HumanMethylation27 BeadChip assessing 27,578 unique CpG sites. Cells were isolated from skin samples donated by young (6-23 years) and elderly (60-73 years) patients undergoing surgical interv...
ORGANISM(S): Homo sapiens 
2012-02-14 | E-GEOD-22595 | biostudies-arrayexpress
TranscriptomicsSurface Topography Enhances Differentiation of Mesenchymal Stem Cells Towards Osteogenic and Adipogenic Lineages
Surface topography impacts on cell growth and differentiation, but it is not trivial to generate homogeneous surface structures and to define the specific morphological parameters of relevance. In this study, we have compared gene expression profiles of mesenchymal stem cells (MSCs) on nanostructure...
ORGANISM(S): Homo sapiens 
2015-08-25 | E-GEOD-61113 | biostudies-arrayexpress
GenomicsReplicative senescence is associated with nuclear reorganization and DNA methylation at specific transcription factor binding sites (MBD-seq)
Primary cells enter replicative senescence after a limited number of cell divisions. This process is associated with reproducible changes in DNA methylation (DNAm) at specific sites in the genome. The mechanism that drives senescence-associated DNAm changes remains unknown and may arise through drif...
ORGANISM(S): Homo sapiens 
2015-03-26 | E-GEOD-59960 | biostudies-arrayexpress
TranscriptomicsReplicative senescence is associated with nuclear reorganization and DNA methylation at specific transcription factor binding sites (RNA-seq)
Primary cells enter replicative senescence after a limited number of cell divisions. This process is associated with reproducible changes in DNA methylation (DNAm) at specific sites in the genome. The mechanism that drives senescence-associated DNAm changes remains unknown and may arise through drif...
ORGANISM(S): Homo sapiens 
2015-03-26 | E-GEOD-59966 | biostudies-arrayexpress
TranscriptomicsReplicative senescence of mesenchymal stem cells causes DNA methylation changes which correlate with repressive histone marks
Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long‐term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony‐ forming unit (CFU‐f) frequency and the differentiation potential ...
ORGANISM(S): Homo sapiens 
2011-10-27 | E-GEOD-26519 | biostudies-arrayexpress
TranscriptomicsMatrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [gene expression profiling]
Matrix elasticity influences differentiation of mesenchymal stem cells (MSCs) but it is unclear if these effects are only transient - while the cells reside on the substrate - or if they reflect persistent lineage commitment. In this study, MSCs were continuously culture-expanded in parallel either ...
ORGANISM(S): Homo sapiens 
2014-07-29 | E-GEOD-55867 | biostudies-arrayexpress
TranscriptomicsPluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes [Illumina]
Pluripotent stem cells evade replicative senescence, whereas other primary cells lose their proliferation and differentiation potential after a limited number of cell divisions M-bM-^@M-^S and this is accompanied by specific senescence-associated DNA methylation (SA-DNAm) changes. Here, we investiga...
ORGANISM(S): Homo sapiens 
2012-09-24 | E-GEOD-37066 | biostudies-arrayexpress
TranscriptomicsTGF-beta1-Induced Gene Expression Changes in Primary Murine Hepatocytes
We here compared gene expression profiles of primary murine hepatocytes (mPC) upon stimulation with 1 ng/ml TGF-beta1 for 20 min, 2 hours and 4 hours with untreated cells. Experiments were done in three independent replicates. The goal of this study was to determine genes regulated by TGF-beta1. 12 ...
ORGANISM(S): Mus musculus 
2013-04-10 | E-GEOD-45942 | biostudies-arrayexpress
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