MDA-MB-231luc1 cells were treated for 6h with PPARbd specific agonists, antagonists and control substances. In addition the influence of PPARbd on gene expression was analyzed via siRNA mediated PPARbd depletion.
Genome-wide occupancy of PPARbeta/delta in human myofibroblast (WPMY-1 celline) was studied with ChIP-Seq. Additionally, H3K4 trimethylation and RNA polymerase II status was examined.