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TranscriptomicsTranscription profiling of wild type and foxA1 null mice
The aim of this experiment was to use microarray analysis to examine the phenotype of the foxA1 (HNF3alpha) null mouse. foxA1 has a central role in the regulatory control of islet genes essential for glucose homeostasis in vivo. Previous studies have shown that the foxA1 null mouse demonstrates sev...
ORGANISM(S): Mus musculus 
2007-09-12 | E-CBIL-37 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of HNF4 alpha null mice to investigate the phenotype of dis-regulated insulin secretion and abnormal beta cell growth
The aim of this experiment was to use microarray analysis to examine the phenotype of dis-regulated insulin secretion and abnormal beta cell growth in HNF4 alpha null mice. These mice show impaired glucose tolerance and elevated fasting and fed plasma insulin levels. Rana Gupta from Klaus Kaestner...
ORGANISM(S): Mus musculus 
2006-09-05 | E-CBIL-6 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Foxa2 knock-outs in mice to better define the role of this gene in glucose homeostasis
Study to further characterize the genetic and functional consequence of the beta cell-specific ablation of Foxa2 (hepatocyte nuclear factor 3 beta, HNF-3b) in mice to better define the role of this gene in glucose homeostasis. The study involved 8 two-channel assays on UPenn Mouse PancChip 4.0, each...
ORGANISM(S): Mus musculus 
2006-06-30 | E-CBIL-13 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat insulinoma (INS-1 derived) cells grown in lipid culture
This experiment was designed to study the effects of lipid culture on INS-1 derived glucose-responsive 832/13 cells. After 48-hr culture with lipids, a dramatic decrease in glucose-responsiveness is observed. In order to detect some earlier changes, RNA was also collected at earlier time points (1...
ORGANISM(S): Rattus norvegicus 
2007-09-11 | E-CBIL-34 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat insulinoma (INS-1 derived) cell lines that vary in their glucose responsiveness
The aim of this study was to identify the gene expression changes associated with glucose responsiveness in Rat INS-1 derived cell lines. RNA was prepared from cell lines which were shown to be highly glucose responsive and also lines which were shown to be poorly glucose responsive. Dr. Chris New...
ORGANISM(S): Rattus norvegicus 
2007-09-11 | E-CBIL-35 | biostudies-arrayexpress
TranscriptomicsFoxA1 regulates sweat secretion through Best2 and Nkcc1 ion transporters
Body temperature is maintained in a narrow range in mammals, primarily controlled by sweating. In humans, the dynamic thermoregulatory organ, comprised of 2-4 million sweat glands distributed over the body, can secrete up to 4 liters of sweat per day1, thereby making it possible to withstand high te...
ORGANISM(S): Mus musculus 
2012-01-11 | E-GEOD-32347 | biostudies-arrayexpress
GenomicsThe Nucleosome Map of the Mammalian Liver
We report the genome-wide map of nucleosome positions in the mouse liver, with emphasis on transcriptional start sites, CpG islands, Foxa2 binding sites, and their correlation with gene expression. Despite the heterogeneity of liver tissue, we could clearly discern the nucleosome pattern of the pred...
ORGANISM(S): Mus musculus 
2011-04-30 | E-GEOD-26729 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse pancreatic islets on day 14.5 of pregnancy
The inability of the beta-cell to meet the demand for insulin brought about by insulin resistance leads to type 2 diabetes. In adults, beta-cell replication is one of the mechanisms thought to cause the expansion of beta-cell mass. Efforts to treat diabetes require knowledge of the pathways that dri...
ORGANISM(S): Mus musculus 
2010-06-26 | E-MTAB-120 | biostudies-arrayexpress
GenomicsChIP-Seq of human normal pancreatic islets with anti-histone antibodies to analyse histone modifications
This experiment used ChIP-seq technology to create a genome-wide profile of histone marks in normal human pancreatic islets. In the current work we analyzed two histone marks associated with gene expression (H3K4me3, H3K4me1) and marks associated with gene repression(H3K27me3). Each mark was anayze...
ORGANISM(S): Homo sapiens 
2010-02-18 | E-MTAB-189 | biostudies-arrayexpress
TranscriptomicsSpontaneous pancreatitis caused by tissue-specific gene ablation of Hhex in mice
Background & Aims: Perturbations in pancreatic ductal bicarbonate secretion often result in chronic pancreatitis. Although the physiological mechanism of ductal secretion is known, its transcriptional control is not well characterized. Here, we investigate the role of the transcription factor Hemato...
ORGANISM(S): Mus musculus 
2015-11-20 | E-GEOD-63526 | biostudies-arrayexpress
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