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ProteomicsDISCO-MS: Proteomics of spatially identified tissues in whole organs and organisms
Spatial molecular profiling of complex tissues is essential to investigate cellular function in physiological and pathological states. However, methods for molecular analysis of biological specimens imaged in 3D as a whole are lacking. Here, we present DISCO-MS, a technology combining whole-organ/is...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-12-23 | PXD034027 | Pride
Disco-ms Disci-bot 
ProteomicsProteomic profiling of FAC-sorted ex vivo and in vitro expanded Sca-1+cells derived from murine skeletal muscle
Stem cells antigen-1 (Sca-1) is an 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein that has widely been used for isolation of murine hematopoietic stem cells (HSCs). However, recently several studies have reported the expression of this protein in non-HSCs such as the liver,...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-21 | PXD022247 | Pride
Regenerative stem cells Stem cells proteomics Stem cell antigen-1 (sca-1) Cell therapy Mass spectrometry-based proteomics 
ProteomicsIntegrating Machine Learning-Enhanced Immunopeptidomics and SARS-CoV-2 Population-Scale Analyses Unveils Novel Antigenic Features for Next-Generation COVID-19 Vaccines
Next-generation T-cell-directed vaccines for COVID-19 aim to induce durable T-cell immunity against circulating and future hypermutated SARS-CoV-2 variants. Mass Spectrometry (MS)based immunopeptidomics holds promise for guiding vaccine design, but computational challenges impede the precise and unb...
ORGANISM(S): Homo sapiens (Human) 
2024-10-14 | PXD052187 | Pride
Immunopeptidomics T-cell epitope Mass spectrometry Sars-cov-2 Covid-19 Next-gen vaccines 
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